_____________________________________________________________________________________________________ *Corresponding author: E-mail: uf.mohammed@fubk.edu.ng; Euro. J. Med. Plants, vol. 34, no. 4, pp. 37-44, 2023 European Journal of Medicinal Plants Volume 34, Issue 4, Page 37-44, 2023; Article no.EJMP.88502 ISSN: 2231-0894, NLM ID: 101583475 Production and Physicochemical Analysis of Glycerol Produced from the Transesterification Reaction of Locally Processed Fatty Acids Mohammed Umar Faruk a* , Ojemuyide Susan Olayemi a and Mohammed Ali Zanna a a Department of Pure and Industrial Chemistry, Faculty of Sciences, Federal University, Birnin Kebbi, Nigeria. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/EJMP/2023/v34i41134 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/88502 Received: 02/05/2022 Accepted: 04/07/2022 Published: 03/05/2023 ABSTRACT The study uses the transesterification reaction method to extract glycerol from three locally processed fatty acids namely; palm oil, palm kernel and shea butter. The glycerols extracted from the three fatty acids were subjected to physicochemical tests to determine if their properties are in conformity with the reported standard values. Results of the physical properties of the substances tested show that the values were in close agreement with the results of the standard values and the results reported impervious literatures. As such, the investigation concluded that the three fatty acids contain appreciable quantities of crude glycerol and can thus serve as a source for natural glycerol whose properties can be compared with standard glycerin products. Original Research Article