~ 3529 ~ The Pharma Innovation Journal 2023; 12(7): 3529-3533 ISSN (E): 2277-7695 ISSN (P): 2349-8242 NAAS Rating: 5.23 TPI 2023; 12(7): 3529-3533 © 2023 TPI www.thepharmajournal.com Received: 01-05-2023 Accepted: 07-06-2023 Bhawana Mathur Department of Plant Molecular Biology and Genetic Engineering, Acharya Narendra Deva University of Agriculture & Technology Kumarganj, Ayodhya, Uttar Pradesh, India NA Khan Department of Plant Molecular Biology and Genetic Engineering, Acharya Narendra Deva University of Agriculture & Technology Kumarganj, Ayodhya, Uttar Pradesh, India DK Dwivedi Department of Plant Molecular Biology and Genetic Engineering, Acharya Narendra Deva University of Agriculture & Technology Kumarganj, Ayodhya, Uttar Pradesh, India Adesh Kumar Department of Plant Molecular Biology and Genetic Engineering, Acharya Narendra Deva University of Agriculture & Technology Kumarganj, Ayodhya, Uttar Pradesh, India Ashish Kumar Department of Plant Molecular Biology and Genetic Engineering, Acharya Narendra Deva University of Agriculture & Technology Kumarganj, Ayodhya, Uttar Pradesh, India Rahul Maurya Department of Plant Molecular Biology and Genetic Engineering, Acharya Narendra Deva University of Agriculture & Technology Kumarganj, Ayodhya, Uttar Pradesh, India Corresponding Author: Bhawana Mathur Department of Plant Molecular Biology and Genetic Engineering, Acharya Narendra Deva University of Agriculture & Technology Kumarganj, Ayodhya, Uttar Pradesh, India Estimation of enzymatic and non-enzymatic activity of rapeseed-mustard against Alternaria blight Bhawana Mathur, NA Khan, DK Dwivedi, Adesh Kumar, Ashish Kumar and Rahul Maurya Abstract An experiment was conducted with 20 rapeseed mustard varieties Rohini, Maya, Giriraj, NDRE-7, NDRS-9-1NDYR-8,Vardan, Vaibhav, Pusa M-27, NDRE-1-11-1, Pusa Gold-45, NRCHB-101, RH-479 Pusa M-24, Kranti, NDR-8501, RH 406, NRCDR-2, RGN-48 and Basanti at Student’s instructional farm of Acharya Narendra Deva University of Agriculture and Technology Kumarganj Ayodhya (U. P.) This study evaluated the enzymatic antioxidant and non-enzymatic antioxidant activity in healthy leaves and leaves exposed to Alternaria blight (Infected), which differed in phenol content, catalase activity and peroxidase activity. The content and activity of phenol, catalase, peroxidase were used parameter to identify the resistant genotype for breeding programmes. The finding revealed that maximum phenol content was recorded in Kranti, whereas maximum catalase activity and peroxidase activity was observed in varieties NDRS-9-1 and Kranti while minimum phenol content, catalase activity and peroxidase activity was observed Rohini in both infected and non-infected leaves of rapeseed mustard varieties. Keywords: Rapeseed-mustard, alternaria blight, phenol, catalase and peroxidase Introduction Rapeseed-mustard crops in India comprises of traditionally grown indigenous species, namely toria (Brassica rapa L. var. toria), brown sarson (Brassica rapa L. var. brown sarson), yellow sarson (Brassica rapa L. var. yellow sarson), Indian mustard (Brassica juncea), and black mustard (Brassica nigra), which have been grown since about 3,500 BC along with non- traditional species like gobhi sarson (Brassica carinata) and Ethiopian mustard or Karan rai (Brassica carinata). The country witnessed yellow revolution through a phenomenal increase in production and productivity from 2.68 MT and 650 kg/ha in 1985-86 to 6.96 MT and 1022 kg/ha in 1996- 1997, respectively. Despite these achievements, there is still a gap between production potential and actual realization (Shekhawat et al., 2012) [7] . India grows annual oilseeds on an area of over 25.74 million hectares, producing 30.55 million tonnes, with a productivity of around 1188 kg per hectare for the quinquennium ending (QE) 2019-20. Among these, Alternaria blight of rapeseed mustard caused by Alternaria brassicae (Berk.) Sacc. is a serious problem with huge economic consequences. This is one of the most serious and devasting disease of mustard under normal conditions. Alternaria is a completely detrimental pathogen causing a widespread destruction in vegetables and other economically important crops. The best and inexpensive way to control Alternaria blight disease of rapeseed-mustard is to use resistant varieties. Alternaria brassicae species have capability to survive in seeds for numerous months at different temperatures and relative humidity. The plants peroxidases have been involved in many defense-related processes, including the allergic responses, lignification, synthesis of phenolics, glycoprotein, suberization and phytoalexin production. Cell often use catalase to quickly breakdown hydrogen peroxide into less reactive gaseous oxygen and water molecules so as not affect the cell (Bolwell and Wojtaszek, 1997) [2] . Material and Methods The study was carried out with 20 rapeseed mustard varieties, namely Rohini, Maya, Giriraj, NDRE-7, NDRS-9-1NDYR-8,Vardan, Vaibhav, Pusa M-27, NDRE-1-11-1, Pusa Gold-45,