COLLABORATI ON OF HI STOI NCOMPATI BLE T AND B LYMPHOCYTES USI NGCELLS FROMTETRAPARENTAL BONE MARROWCHI MERAS BY H . voN BOEHMER, L . HUDSON, AND J . SPRENT* ( Fr omt he Basel I nst i t ut e f or I mmunol ogy, CH- 4058 Basel , Swi t zerl and) Experi ment s have been r epor t ed suggest i ng t hat under condi t i ons wher e an al l ogenei c ef f ect coul d not be demonst rat ed, pri med hi st oi ncompat i bl e T and B cel l s di d not cooper at e in a humoral i mmune r esponse ( 1, 2) . By cont r ast sem i al l ogenei c T and B cel l s wer e f ound to cooper at e . The aut hor s t her ef or e concl uded t hat in or der to obt ai n ef f i ci ent T- B col l abor at i on t he cel l s concer ned mus t shar e det er m i nant s coded f or by t he H- 2- gene compl ex, mos t l i kel y t he I r egi on ( 3) . In di f f er ent experi ment s Becht hol et al . ( 4, 5) demonst rat ed t hat al l opheni c m i ce, pr oduced by t he f usi on of embr yos of st r ai ns gi vi ng a hi gh and l ow r esponse to t he synt het i c ant i gen pol y- L( Tyr , Gl u) - pol y- D, L- Al a- - pol y- L- Lys ( TGAL) , ' pr oduced TGAL- speci f i c ant i body of l ow r esponder al l ot ype . The au- t hor s i nt er pr et ed t hi s f i ndi ng as an i ndi cat i on of col l abor at i on bet ween hi st oi n- compat i bl e hi gh r esponder T cel l s and l ow r esponder B cel l s . While t hi s i nt er pr e- t at i on may be cor r ect , di r ect evi dence has not been pr esent ed to suppor t t he not i on . It coul d be ar gued, f or exampl e, t hat in al l opheni c m i ce l ow r esponder T cel l s show a hei ght ened r esponse to TGAL . While t he mechani sm of such a conver si on is unknown t he possi bi l i t y shoul d be consi der ed unt i l di spr oven by experi ment al dat a . It is obvi ousl y i mport ant to know whet her t he pr esence of cer t ai n det er m i- nant s on al l ogenei c T and B cel l s pr event T- B col l abor at i on under al l ci rcum- st ances, or whet her t hi s is onl y so when t he cel l s ar e not mut ual l y t ol er ant . We have st udi ed t he col l abor at i on of hi st oi ncompat i bl e T and B l ymphocyt es in r esponse to sheep er yt hr ocyt es ( SRBC) in an adopt i ve t r ansf er syst em usi ng pri med T cel l s f r om t et r apar ent al m i ce and pri med B cel l s f r om bot h nor mal and chi meri c m i ce . In pr evi ous r epor t s we have shown t hat T cel l s f r om t et r apar en- t al bone mar r ow chi meras ( TBMC) ar e speci f i cal l y unr esponsi ve to t he det er m i- nant s of t he H- 2 compl ex of t he host in t he m i xed l eukocyt e r eact i on ( MLR) as wel l as in cel l -medi at ed l ymphol ysi s ( CML) ( 6, 7) . The dat a pr esent ed her e suggest s t hat pri med T cel l s ar e abl e to cooper at e with al l ogenei c B cel l s to whi ch t hey have been t ol er i zed in a chi meri c envi r onment . * Pr esent addr ess : Depart ment of Zool ogy, Uni ver si t y Col l ege, London, Engl and . ' Abbr evi at i ons used in t hi s paper : CML, cel l -medi at ed l ymphol ysi s ; LN, l ymph node ; MLR, m i xed l eukocyt e r eact i on ; PFC, pl aque- f or m i ng cel l ; TBMC, t et r apar ent al bone mar r ow chi meras ; TDL, t hor aci c duct l ymphocyt es ; TGAL, pol y- L( Tyr , GI u) - pol y- D, L- Al a- - pol y- L- Lys . THE J OURNAL OF EXPERI MENTAL MEDI CI NE - VOLUME 142, 1975 989 Downloaded from http://rupress.org/jem/article-pdf/142/4/989/1657243/989.pdf by guest on 25 July 2023