© The Author(s) Publisher: Ilam University of Medical Sciences Journal of Ilam University of Medical Sciences: Volume 29, Issue 6, 2022 Evaluation of Anti-Cancer Effects of Caspian Cobra (Naja naja oxiana) Snake Venom in Comparison with Doxorubicin in HeLa Cancer Cell Line and Normal HFF Fibroblast Fatemeh Javani Jouni 1 , Jaber Zafari 2 , Elaheh Shams 3 , Parviz Abdolmaleki 4 , Ali asghar Rastegari 5 * 1. Dept of Biomedical Engineering, Faculty of Health, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran 2. Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran 3. Behbahan Faculty of Medical Sciences and Health Services, Behbahan, Iran 4. Dept of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran 5. Dept of Molecular and Cell Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran Article Info A B S T R A C T Article type: Research article Introduction: Cancer is the leading cause of death in most countries. There are several methods used to treat cancer. Doxorubicin is one of the most important chemotherapy drugs that has several side effects, such as infertility, hyperuricemia, neuropathy, and cardiomyopathy. This study aimed to evaluate the anti-cancer effects of Naja naja oxiana in comparison with doxorubicin in Hela (Human cervical cancer) and HFF (Human foreskin fibroblast) cell line. Material & Methods: Hela and normal fibroblast cancer cell lines were exposed to different concentrations (1, 10, 50, 100, and 500 μg/ml) of snake venom and doxorubicin. The MTT method was used to evaluate the IC50 (Inhibitory Concentration) for toxins and drugs. Finally, the results were analyzed using SPSS software (version 19). Findings: The results show that with increasing concentration and time of treatment with snake venom and doxorubicin, the percentage of Hela and fibroblasts living cells decreases. The highest decrease in the percentage of the viable cells was observed in the Hela cancer cell line treated with a concentration of 500 μg/ml snake venom for 48 h. Discussion & Conclusion: Snake venom can have a significant inhibitory effect on the percentage of living Hela cancer cells in comparison with doxorubicin. Keywords: Doxorubicin, Human cervical cancer cells, Snake venom Article History: Received: 08 June 2021 Revised: 26 June 2021 Accepted: 29 November 2021 * Correspondence to: Ali Asghar Rastegari Dept of Molecular and Cell Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran Email: aarastegari@gmail.com ➢ How to cite this paper Javani Jouni F, Zafari J, Shams E, Abdolmaleki P, Rastegari A. Evaluation of Anti-Cancer Effects of Caspian Cobra (Naja naja oxiana) Snake Venom in Comparison with Doxorubicin in HeLa Cancer Cell Line and Normal HFF Fibroblast. Journal of Ilam University of Medical Sciences. 2022;29(6): 20-27. [ DOI: 10.52547/sjimu.29.6.20 ] [ Downloaded from sjimu.medilam.ac.ir on 2022-05-29 ] 1 / 8