World Journal of Cancer and Oncology Research, 2023, 3, 727 www.scipublications.org/journal/index.php/wjcor DOI: 10.31586/wjcor.2023.727 DOI:https://doi.org/10.31586/wjcor.2023.727 World Journal of Cancer and Oncology Research Review Article Anti-Cancer and Anti-Fungal Activities of Calotropis procera: a Narrative Review Shafqat Rasul 1 , Abid Rashid 2 , Muhammad Akram 1,* , Umme Laila 1 , Rida Zainab 1 , Muhammad Talha Khalil 1 , Hadji Seydou Mbaye 3 , Mourad Ben Said 4,5 , Hanan Sneed Alharbi 6 , Hanadi Husni Alabed 7 , Mahmoud Abdel Hameed Shahin 8 1 Department of Eastern Medicine, Government College University Faisalabad, Pakistan 2 Faculty of Medical Sciences, Government College University Faisalabad, Pakistan 3 BCNet International Working Group, IARC/WHO, Dakar, Senegal 4 Service de MicrobiologieetImmunologie, EcoleNationale de MédecineVétérinaire, Univ. Manouba, 2020 SidiThabet, Tunisia 5 Institut Supérieur de Biotechnologie de SidiThabet, Département des Sciences Fondamentales, Univ. Manouba, SidiThabet, Tunisia 6 Nursing department, Ohud hospital, Madinah, KSA 7 Education department, Gama Hospital, Khobar, KSA 8 Associate Professor, Nursing Department, Prince Sultan Military College of Health Sciences, Dhahran, KSA *Correspondence: Dr. Muhammad Akram (makram_0451@hotmail.com) Abstract: Calotropis procera, a medicinally important plant found in Asia, was explored for its anticancer and antibacterial properties in this study. The leaves of C. procera were extracted using methanol and FTIR and UV-VIS spectrophotometry were used to characterize them. Using the MTT assay and the disc diffusion test, the extract was examined for anticancer activity against the MCF7 breast cancer cell line and antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). The methanolic fraction of C. procera was found to be efficient against the MCF7 cell line and dramatically suppressed MRSA growth. The metabolic fraction of C. procera leaves is important in suppressing the growth of the MCF7 cell line, and it has the potential to be an effective antibacterial agent, according to our findings. The implications of Calotropis procera for all healthcare professionals including oncologists, physicians, pharmacists, nurses, and nutritional therapists are significant. With the increasing incidence of cancer and antibiotic-resistant bacterial infections, there is a growing need for new, effective, and safer herbal treatments. Keywords: Calotropis procera, Medicinal uses, Pharmacological uses, anti-Cancer and anti-fungal activities 1. Introduction Microbes are tiny creatures that can live alone or in a group. Since antiquity, the probable presence of unseen microbial life was assumed. The microscopic study of microorganisms began with their scrutiny by Antonie van Leeuwenhoek in the 1670s. In the 1850s, Louis Pasteur also discovered the microorganism which causes food to decompose, thus exposing the theory of impulsive generation. Robert Koch discovered in the 1880s the microorganism, which causes the diseases of cholera, tuberculosis, and anthrax. Microorganisms include all extremely diverse single-celled organisms. In human culture and health, microbes play an important role in different ways such as food fermentation, processing, fuel production, enzymes, and other bioactive compounds. The human microbiota including the essential intestinal flora of the human body made up of How to cite this paper: Rasul, S., Rashid, A., Akram, M. ., Laila, U., Zainab, R., Khalil, M. T., Mbaye, H. S., Said, M. B., Alharbi, H. S., Alabed, H. ., & Shahin, M. (2023). Anti-Cancer and Anti-Fungal Activities of Calotropis procera: a Narrative Review. World Journal of Cancer and Oncology Research, 2(2), 102–121. Retrieved from https://www.scipublications.com/jou rnal/index.php/wjcor/article/view/72 7 Academic Editor: Mohamed Kotb El-Sayed Received: xx xx, xx Accepted: xx xx, 2023 Published: xx xx, 2023 Copyright: © 2023 by the authors. Submitted for possible open-access publication under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses /by/4.0/).