SHORT REPORT The article was published by Academy of Chemistry of Globe Publications www.acgpubs.org/RNP © Published 12/24/2016 EISSN:1307-6167 Rec. Nat. Prod. 11: 2 (2017) 223-228 Anti-urease Secondary Metabolites from Seriphidium quettense Muhammad Imran Tousif 1, 2 , Naheed Riaz 1 , Mamona Nazir 3, 4 , Shabir Ahmad 5 , Muhammad Saleem 1* , Abdul Jabbar 1 , Muahammad Ashraf 1 and Rasool Bakhsh Tareen 6 1 Department of Chemistry, Baghdad-ul-Jadeed Campus, The Islamia University of Bahawalpur, 63100 Bahawalpur, Pakistan 2 Department of Chemistry, Dera Ghazi Khan Campus, University of Education Lahore, Dera Ghazi Khan, Pakistan 3 Department of Chemistry, The Government Sadiq College Women University, Bahawalpur, 63100 Bahawalpur, Pakistan 4 Institute for Pharmaceutical Biology, Nussalle 6, University of Bonn, 53115 Bonn, Germany 5 Department of Chemistry, Post-Graduate College, University Road Bahawalpur, 63100 Bahawalpur, Pakistan 6 Department of Botany, University of Baluchistan, Quetta, Pakistan (Received February 3, 2016; Revised September 27, 2016; Accepted September 27, 2016,) Abstract: Ethyl acetate layer of the methanolic extract of Seriphidium quettense was subjected to silica gel column chromatography to isolate one new; seriphiloid (1), and four known compounds; ilicic acid (2), 6- hydroxy-8(10)-oplopen-14-one (3), 2-(4-hydroxyphenyl)-5,6,7-trimethoxy-4H-chromen-4-one (4) and 2-(3,4- dihydroxyphenyl)-5,6,7-trimethoxy-4H-chromen-4-one (5). The chemical structure of the new isolate was established with the help of 1D, 2D NMR techniques and high resolution mass spectrometry. Known compounds were identified because of 1D NMR and mass spectrometric analysis and in comparison with the literature values. Compounds 1-5 were evaluated for their acetylcholinesterase, butyrylcholinesterase, -glucosidase and urease inhibitory activities. Most of the metabolites were found inactive; however, compounds 2 and 3 showed good antiurease activity with IC 50 value 21.5±0.1 and 20.8±0.1 µg/ mL, respectively. Keywords: Seriphidium quettense; secondary metabolites; structure elucidation; enzyme inhibition. © 2016 ACG Publications. All rights reserved. 1. Plant Source Seriphidium quettense of the plant family Asteraceae is a woody shrub growing in Hazarganji, Baluchistan, Pakistan [1, 2]. The chromatographic purification of the methanolic extract of this plant yielded one new compound seriphiloid (1) and four known metabolites; ilicic acid (2), [3] 6- hydroxy-8(10)-oplopen-14-one (3), [4, 5] 2-(4-hydroxyphenyl)-5,6,7-trimethoxy-4H-chromen-4-one (4) [5] and 2-(3,4-dihydroxyphenyl)-5,6,7-trimethoxy-4H-chromen-4-one (5) [6] (Figure 1). * Corresponding author: E- Mail: m.saleem@iub.edu.pk (M. Saleem), Phone +92-343-7007885