~ 1108 ~ International Journal of Chemical Studies 2018; 6(2): 1108-1111 P-ISSN: 2349–8528 E-ISSN: 2321–4902 IJCS 2018; 6(2): 1108-1111 © 2018 IJCS Received: 16-01-2018 Accepted: 18-02-2018 Prerana B Abhang Department of Plant Pathology Post Graduate Institute, Dr. Panjabrao Deshmukh Krishi Vidyapeeth Akola, Maharashtra, India MV Totawar Department of Plant Pathology Post Graduate Institute, Dr. Panjabrao Deshmukh Krishi Vidyapeeth Akola, Maharashtra, India Maduri M Katkar Department of Plant Pathology M.P.K.V. Rahuri. Maharashtra, India Priya C Atram Department of Plant Pathology Post Graduate Institute, Dr. Panjabrao Deshmukh Krishi Vidyapeeth Akola, Maharashtra, India SS Mane Department of Plant Pathology Post Graduate Institute, Dr. Panjabrao Deshmukh Krishi Vidyapeeth Akola, Maharashtra, India Correspondence Prerana B Abhang Department of Plant Pathology Post Graduate Institute Dr. Panjabrao Deshmukh Krishi Vidyapeeth Akola, Maharashtra, India Efficacy of fungicides botanicals bioagents against Xanthomonas axonopodis pv. citri Prerana B Abhang, MV Totawar, Maduri M Katkar, Priya C Atram and SS Mane Abstract Citrus canker disease of acid lime caused by Xanthomonas axonopodis pv. citri is an important disease in many parts of MH region. In vitro experiment were conducted to study efficacy of fungicides botanicals bioagents against Xanthomonas axonopodis pv. citri. The growth characteristics of all the isolates were studied on nutrient agar medium. All seven isolates were found Gram –ve, short rod and positive for biochemical tests viz. KOH, catalase test, starch hydrolysis, Indole production, acid and gas production and gelatein liquefacation. The efficacy of bioagents, botanicals and chemicals was studied by paper disc method. The Copper oxychloride (0.2%) + streptomycin sulphate (200 ppm) was found significantly effective in inhibiting growth of bacteria. Keywords: fungicides, botanicals, Xanthomonas axonopodis pv Introduction Citrus is an important value as fruit crop. Present day citrus is delectable, juicy, and seedless is of great nutritional significance as well (Khan et al., 1992) [1, 5] . Additionally, it possesses enormous therapeutic qualities (Chaudhry et al., 1992) [1] . Citrus is a member of Rutaceae family and grown in varying densities in countries with tropical or subtropical climates. Citrus plant is attacked by a number of diseases like citrus canker, gummosis, citrus decline, citrus tristeza virus, greening, etc. Citrus canker is caused by “Xanthomonas campestris pv. citri” that is probably the worst enemy to citrus plants (Sahi et al., 2007). Citrus canker disease is of regular occurrence on several citrus cultivars in varying degrees of incidence depending on the climatic conditions. The bacterium, Xanthomonas causes different symptoms ranging from pustules to necrotic lesions consisting of erumpent corky tissue surrounded by water soaked tissues and yellow halo on leaves, stems and fruits (Zekri et al., 2005; Graham et al., 2004; Das, 2003) [7, 2] . Xanthomonas axonopodis pv. citri is a rod shaped, gram negative bacterium with single polar flagellum. Material and Methods Collection, isolation and maintenance of bacterial strains. The citrus canker diseased sample were collected from different district of Maharashtra state viz. Akola, Warora, Nagpur, Parbhani, Sangli, Rahuri, Pune (Table 1). A total of seven isolates of pathogen were obtained from infected leaves, twigs of acid lime showing typical symptoms of citrus canker. These isolates were isolated by tissue isolation method. A bacterial suspension of each specimen was then cultured on NA medium. Following incubation, colonies similar to Xanthomonas were maintained on NA medium at room temperature by adopting subsequent subculturing at periodical, regular intervals. Three days old cultures were used for further studies. Table 1: List of isolates of Xanthomonas axonopodis pv. citri Sr. No Location District Code no. 1 AICRP on fruits Dr. P.D.K.V. Akola Akola Xac1 2 Warora Chandrapur Xac2 3 Nagpur Nagpur Xac3 4 Parbhani Parbhani Xac4 5 Sangli Sangli Xac5 6 M.P.K.V.Rahuri Ahmednagar Xac6 7 C.O.A.Pune 05 Pune Xac7