© IJARW | ISSN (O) - 2582-1008 August 2023 | Vol. 5 Issue. 2 www.ijarw.com IJARW1946 International Journal of All Research Writings 53 PRODUCTION OF ACETOBACTER XYLINUM MICROBIAL STARTER WITH LIME AND FOOD VINEGAR ADDITIONAL Aulia Andhikawati 1 , Nora Akkbarsyah 2 , Sari Usih 3 1,2 Tropical Marine Fisheries Study Program, PSDKU UNPAD Pangandaran, Pangandaran Regency, West Java, Indonesia 3 Business Administration Study Program, PSDKU UNPAD Pangandaran, Pangandaran Regency, West Java, Indonesia ABSTRACT Acetobacter xylinum are obligate aerobic bacteria that metabolize mainly glucose which is used in the synthesis of cellulose. The floating matrix will allow the cells to rise to the surface of the media where oxygen is abundant. The purpose of this study was to determine the addition of different acids to A. xylinum starter. The method used to grow starter A. Xylinum consisted of two different treatments, namely the addition of lime and 5% Food Vinegar. This research was tested descriptively. Preparation of microbial starter Acetobacter xylinum using the addition of lime juice gives faster changes every time compared to the 5% Food Vinegar treatment. In the fermentation with the addition of white coating lime and color changes occurred on the second day, while the 5% Food Vinegar treatment occurred on the sixth day. Keyword: Acetobacter xylinum, fermentation, lime, food Food Vinegar 1. INTRODUCTION According to[1], The definition of a starter is a number of additional ingredients that can be used during the initial phase of the fermentation process. Starter is a process that is intended to grow certain microbes in a substrate or medium that can make these microbes grow in a certain purpose and process. The process of growing bacteria using this starter method has conditions that must be known such as the medium where it must be clean, sterile, superior, the materials used are in accordance with procedures, stable during the process, and also not harmful pathogens. According to [2] said that, the conditions for making a starter are safe, and can also withstand harmful bacteria in the process of making a starter Microorganisms in the starter manufacturing process will grow and reproduce simultaneously and are active. This growing microbe will change the material in the starter process into the expected material [3]. Environmental factors and nutrient content in the process of making this starter will affect the growth rate of microbes. The fermentation process will run if it uses the benefits of glucose as an energy source. The nutrients in the substrate or medium will decrease as the fermentation process progresses and the duration of the process. The large number of microbes that grow and reproduce will result in competition and will end in the death phase [1]. Acetobacter xylinum microbe is one of the microbes that will often be encountered in the field of fisheries. This microbe has benefits such as making nata which is produced from solulose fermented by Acetobacter xylinum. Acetobacter xylinum bacteria can produce extracellular enzymes that can polymerize glucose into thousands of fiber or cellulose homopolymer chains. There is an easy way to get specific and structured Acetobacter xylinum microbes with bacterial culture. There are various types of fermentation, one of which is starter fermentation. According to[4] explained that, starter fermentation is a fermentation that is processed in foodstuffs with the addition of microorganisms that will play an active role in changing the fermented material into the expected product. These microorganisms will multiply. In this case, we can use these microorganisms as a role in production. The role that is utilized in this microorganism is the metabolic activity of the microorganism.