[Sulieman et. al., Vol.4 (Iss.7): July, 2016] ISSN- 2350-0530(O) ISSN- 2394-3629(P)
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Science
EVALUATION OF SOME MICROSCOPIC TECHNIQUES FOR
DETECTING BILHARZIA AND INTESTINAL PARASITES
Azzam Afifi
1
, Abdel-Aziz A. Ahmed
2
, Yassir Sulieman
*3
, Mohamed A. Zakaria
4
,
Theerakamol Pengsakul
5
1
Department of Zoology, Faculty of Science and Technology, Omdurman Islamic University,
SUDAN
2
Department of Zoology, Faculty of Science, University of Khartoum, SUDAN
*3
Department of Zoology, Faculty of Science and Technology, University of Shendi, SUDAN
4
Department of Biology, Faculty of Education, University of Nyala, SUDAN
5
Faculty of Medical Technology, Prince of Songkla University, Hat Yai, Songkhla 90110,
THAILAND
DOI: https://doi.org/10.29121/granthaalayah.v4.i7.2016.2611
ABSTRACT
The objective of the present study was to evaluate the efficacy of five different diagnostic
techniques (haematouria, macro-haematouria, syringe filtration, vacuum flask filtration and
sedimentation) in the detection of urinary schistosomiasis. Likewise, five techniques (direct
smear, cellophane thick smear, ether-formalin, modified Kato-template and modified Kato-
syringe) techniques used in the detection of intestinal schistosomiasis and other intestinal
parasites were evaluated using fecal samples. The results showed that the most efficient
diagnostic technique for fecal samples is the modified Kato-Katz technique, while the ether-
formalin method was the most sensitive tool for the detection of Schistosoma mansoni,
Enterobius sp. and Ascaris sp. rates of infection and worm burden. The modified Kato
techniques (template and syringe) failed to detect the intestinal protozoa, Giardia lamblia and
Entamoeba histolytica, while the syringe filtration technique was the most powerful urine test.
Keywords:
Microscopic; Diagnosis; Evaluation; Schistosomes; Intestinal parasites.
Cite This Article: Azzam Afifi, Abdel-Aziz A. Ahmed, Yassir Sulieman, Mohamed A. Zakaria,
and Theerakamol Pengsakul, “EVALUATION OF SOME MICROSCOPIC TECHNIQUES
FOR DETECTING BILHARZIA AND INTESTINAL PARASITES” International Journal of
Research – Granthaalayah, Vol. 4, No. 7 (2016): 185-195.
1. INTRODUCTION
There are many different microscopic diagnostic techniques for detecting schistosomes and
intestinal parasites. These techniques vary in their sensitivity in the diagnostic screening of