© 2017, IJCSE All Rights Reserved 45                               Research Paper Volume-5, Issue-3 E-ISSN: 2347-2693 An Automated Skull-Stripping Method by Windowing the Histogram S. Sarkar 1* , A. Mandal 2 , K. Sarkar 3 1* Department of Computer Science & Application, University of North Bengal, Darjeeling, India 2 Department of Computer Science & Application, University of North Bengal, Darjeeling, India 3 Ananda Chandra College, Department of Computer Science, University of North Bengal, Darjeeling, India * Corresponding Author: suvro.nbu@gmail.com, Tel.: +918942921147 Available online at: www.ijcseonline.org Received:26/Feb/2017 Revised: 03/Mar/2017 Accepted: 20/Mar/2017 Published: 31/Mar/2017 AbstractAn automated method for segmentation of Magnetic Resonance (MR) head images into brain and non-brain has been proposed. It combines the strategy used in intensity and morphological skull-stripping methods. The method is very fast and requires no preprocessing of MR images. It is testified on T1-Weighted MR image modality and produces accurate output. Keywords—MRI, Skull-Stripping , ROI, T1-Weighted, Windowing I. INTRODUCTION The human brain is very complex structure composed of Neurons, Glial cells, Neural Stem cells and blood vessels. A key issue in neuro-image e.g. Magnetic Resonance Image, (MRI) analysis is so called skull-stripping, which plays an important role in neuro-image processing, is the task of separating predominant tissues in head, e.g. Gray Matter (GM), White Matter (WM) and Cerebrospinal Fluid(CSF), from the other tissues in head such as Bone, Skin, Muscle, Fat Dura etc. The accuracy of skull-stripping method affects the later stages of neuro-image analysis to a great extent. The measured signal intensity of brain tissue and non-brain tissue can overlap. This overlapping produces ambiguity in separation procedure and thus resulting false positive and false negative identification. This problem is so called non- separability in the context of digital image processing. II. TECHNICAL REVIEW In general, skull-stripping, is a non-trivial task as the acquired MR images are imperfect and are often noisy. The diversity of MR images of brain led to the development of various skull-stripping techniques (BET [1], BSE [2], Bridge Burner [3] and GCUT [4]). Different methods of skull- stripping available in the literature are broadly classified into five categories [5]: i. Mathematical morphology based method. ii. Intensity based method. iii. Deformable surface based method. iv. Atlas based method v. Hybrid method The proposed method is based on the combination of Mathematical morphology and Intensity based methods. So brief explanations of these two categories only are presented here: A. Morphology-based method In this method, two important mathematical and morphological operators are used namely: Erosion and Dilation. In general, the image is first transformed into a binary image then the following operators are applied: i. Erosion: The erosion of a binary image ‘A’ by a structuring element ‘B’ in an Euclidean space ‘E’ is defined as- } | { A B E z B A z = Θ (1) Where B z is the translation of B by the vector z, i.e. E B b z b B z z + = }, | { (2) ii. Dilation: The dilation a binary image ‘A’ , in the Euclidean Space ‘E’, by a structuring element ‘B’ is defined as- } ) ( | { φ = A B E z B A z s (3) Where B s denotes the symmetric of B, that is, } | { B x E x B s - = (4) An appropriate threshold is applied to find the initial Region of Interest (ROI), and then the morphological operators are applied in order to justify the ROI more appropriately to the desired outcome. One of the commonly used methods in this category is discussed by Brummer et al [6]. The method is based on the histogram threshold and morphological operations. Main drawback of such method, in skull- stripping, is that the final output is directly influenced by the parameters such as size and shape of the structural element,