_____________________________________________________________________________________________________ *Corresponding author: E-mail: drambadaskadam@gmail.com; European Journal of Medicinal Plants 31(8): 61-75, 2020; Article no.EJMP.56424 ISSN: 2231-0894, NLM ID: 101583475 In vivo Approach: Potential Diuretic Activity of M. charantia Linn. on Alloxan Induced Albino Wistar Rats in Diabetes Mellitus Umesh B. Wahul 1 , Ambadas S. Kadam 2* , Laxmikant H. Kamble 2 and Ayaz S. Ali 3 1 School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, 431606(MS), India. 2 DSM Arts, Commerce, Science College, Jintur, Parbhani, 431509 (MS), India. 3 Y. B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Aurangabad, 431003(MS), India. Authors’ contributions This work was carried out in collaboration among all authors. Author UW designed the study, performed the statistical analysis, wrote the protocol and wrote the first draft of the manuscript. Authors AK and LK managed the analyses of the study. Author AA managed the literature searches. All authors read and approved the final manuscript. Article Information DOI:10.9734/EJMP/2020/v31i830259 Editor(s): (1) Dr. Patrizia Diana, University of Palermo, Italy. Reviewers: (1) Vivek Kumar Sharma, Government College of Pharmacy Rohru, India. (2) K.D.P.P. Gunathilake, Wayamba University of Sri Lanka, Sri Lanka. (3) Sana Sarfaraz, University of Karachi, Pakistan. Complete Peer review History: http://www.sdiarticle4.com/review-history/56424 Received 01 March 2020 Accepted 06 May 2020 Published 14 May 2020 ABSTRACT The screening of perfect diuretics for non –clinical utility in Diabetes mellitus is a relatively novel approach which gain insight into underlying the pathophysiological processes. This study aim to evaluate the diuretic effect of crude aqueous & alcoholic extract of M. charantia Linn. using Albino Wistar Rat model. The methodology of animal study includes the spectral analysis of Na + , K + , Cl - concentration against the body weight, this was done by spectrophotometry. In this study, the comparative observation of Diuretic activity with standard and extracted compound has shown that the estimated 24‐hour urine contains the Na+-3.82 g, 3.82 g, 3.92 g and K+-1.35 g, 1.39 g, 1.48 gwt. For Vehicle control, Standard drug, and Extracted compound respectively. Which possess the favouring result means from the spot urine were 10.7±7.0 g/24 hand 3.9±2.1 g/24 h, respectively. Coefficients were 0.035, 0.022, 0.046 at (d±2SD=7.07 g, Original Research Article