1164 The Journal of Rheumatology 2005; 32:6
Case Report
Persistent Cryoglobulinemic Vasculitis Following
Successful Treatment of Hepatitis C Virus
JAMES W. LEVINE, CARMEN GOTA, BARRI J. FESSLER, LEONARD H. CALABRESE, and SHELDON M. COOPER
ABSTRACT. There is a well established link between type II mixed cryoglobulinemia (MC) and hepatitis C virus
(HCV) infection, and HCV is believed to be the cause of cryoprotein formation and tissue deposi-
tion. Successful treatment of HCV infection has resulted in resolution of cryoglobulinemia and vas-
culitis. We describe 4 patients who had persistent MC and vasculitis despite successful eradication
of HCV with antiviral therapy. (J Rheumatol 2005;32:1164–7)
Key Indexing Terms:
CRYOGLOBULINEMIA HEPATITIS C VIRUS VASCULITIS
From the Rheumatology and Clinical Immunology Division, University of
Vermont College of Medicine, Burlington, Vermont; Rheumatic and
Immunologic Diseases Division, Cleveland Clinic Foundation, Cleveland,
Ohio; and the Division of Clinical Immunology and Rheumatology,
University of Alabama at Birmingham, Birmingham, Alabama, USA.
J.W. Levine, DO; S.M. Cooper, MD, Rheumatology and Clinical
Immunology Division, The University of Vermont College of Medicine;
C. Gota, MD; L.H. Calabrese, DO, Rheumatic and Immunologic
Diseases, Cleveland Clinic Foundation; B.J. Fessler, MD, Division of
Clinical Immunology and Rheumatology, The University of Alabama at
Birmingham.
Address reprint requests to Dr. S.M. Cooper, The University of Vermont
College of Medicine, Given Building D305, 89 Beaumont Avenue,
Burlington, VT 05405. E-mail: sheldon.cooper@uvm.edu
Accepted for publication January 24, 2005.
Hepatitis C virus (HCV), a worldwide pathogen, is the lead-
ing cause for endstage liver disease and indication for liver
transplant in the United States. HCV is also associated with
a variety of immunologic extrahepatic manifestations, the
best studied of which is the presence of elevated cryoglobu-
lins in the serum. While up to 40%–50% of HCV infected
patients have elevated levels of circulating cryoglobulins
1,2
,
only a small proportion develop vasculitic sequelae in target
organs such as the skin, peripheral nerve, and kidney
3
. There
is strong evidence, particularly from studies of the composi-
tion of the cryoglobulins themselves
2
as well as from
immunohistologic studies of tissues such as skin
4
, that HCV
is a cause of the formation of the cryoprotein and ultimate-
ly its deposition in the target tissues.
The most common type of cryoglobulinemia in patients
with HCV is type II mixed cryoglobulinemia (MC), charac-
terized by the presence of polyclonal Ig in association with
a monoclonal Ig (typically IgM or IgG). Treatment of cryo-
globulinemia is indicated when progressive organ-threaten-
ing disease is present. Prior to the association of MC with
HCV, treatment generally consisted of high dose glucocorti-
coids, cytotoxic agents, and plasmapheresis. While
described as effective, this therapy was often only transient-
ly beneficial
5,6
. However, therapy directed against HCV
with interferon-α (IFN-α) and more recently combined
IFN-α and ribavirin has offered a new strategy for HCV
associated cryoglobulinemia. This therapeutic strategy has
been reported to be successful at achieving remission for
HCV related MC
7-11
.
In virtually all reports of successful treatment of HCV
associated cryoglobulinemic vasculitis there has been a
strong correlation between antiviral response and vasculitic
response
11-13
. Particularly with cutaneous vasculitis, a sus-
tained virologic response has been associated with effective
and enduring remissions. In contrast, patients who either do
not achieve an antiviral effect or who experience a relapse
will have incomplete or transient improvement in the vas-
culitis
7,8,14-17
. Thus recurrence of symptoms attributable to
cryoglobulinemia is paralleled by recurrence of detectable
HCV RNA in those who have received IFN therapy
7
.
We recently identified 4 patients from 3 centers who had
persistent symptomatic cryoglobulinemia despite successful
and sustained HCV eradication with antiviral therapy. Data
from these 4 patients were collected retrospectively and
form the basis of this observational report.
CASE REPORTS
Pertinent details for each case are listed in Table 1.
Patient 1. A 49-year-old man was diagnosed with HCV infection (genotype
2b) in 1999. In May 2001 he developed lower extremity petechiae and was
found to have type II MC with an IgM kappa monoclonal component.
Thrombocytopenia subsequently developed and he was treated with pred-
nisone, which promptly improved the platelet count although petechiae per-
sisted. Before antiviral therapy the viral load by HCV RNA assay (Roche
Amplicor HCV Monitor) was > 850,000 IU/ml. He received a 24-week
course of pegylated IFN-α and ribavirin starting in October 2002. Lower
extremity petechiae resolved and subsequent testing for HCV RNA
(Amplicor) revealed no detectable virus (sensitivity of < 600 IU/ml), but
persistence of cryoglobulins. The cryocrit prior to antiviral therapy was
23% and remained between 10% and 20% throughout the period of obser-
vation. The levels of C4 were < 10 mg/dl (normal 16–38) before and after
antiviral therapy. In July 2003, 4 months after cessation of antiviral thera-
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