Research Article Characterizationof Streptomyces CellSurfacebytheMicrobial AdhesiontoSolventsMethod C.Zanane ,S.Mitro ,D.Mazigh ,S.Lekchiri ,T.Hakim ,M.ElLouali , H.Latrache ,andH.Zahir Industrial and Surface Engineering, Research Team of Bioprocesses and Biointerfaces, Faculty of Sciences and Techniques, Sultan Moulay Slimane University, Beni Mellal, Morocco Correspondence should be addressed to H. Latrache; latracheh@yahoo.fr and H. ahir; hafdazahir@yahoo.com Received 13 February 2023; Revised 29 March 2023; Accepted 12 April 2023; Published 11 May 2023 Academic Editor: Karl Drlica Copyright © 2023 C. anane et al. Tis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Te cell surface physicochemical properties of Streptomyces should infuencing the dispersal and adsorption of spores and hyphae in soil and should conditioning there interactions with organic or metal substances in the bioremediation of contaminated environment. Tese properties are concerning surface hydrophobicity, electron donor/acceptor, and charge surface. Todate,only hydrophobicity of Streptomyces wasstudiedbycontactanglemeasurementsandmicrobialadhesiontohydrocarbons(MATH).In this work, we studied the electron donor/acceptor character of the Streptomyces cell surface in two ionic strength 10 −3 M and 10 −1 MofKNO 3 . Tus, to facilitate the characterisation of the surfaces of microbial cells, we used a simple, rapid, and quantitative technique, the microbial adhesion method to solvents (MATS), which is based on the comparison of the afnity of microbial cells for a monopolar solvent with a polar solvent. Te monopolar solvent can be acid (electron acceptor) or basic (electron donor), but both solvents should have a surface tension similar to that of the Kifshitz van der Waals components. At the signifcant ionic strength of the biological medium, the electron donor character is well expressed for all 14 Streptomyces strains with very signifcant diferences among them ranging from 0% to 72.92%. When the cells were placed in a solution with a higher ionic strength, we were able to classify the donor character results into three categories. Te frst category is that the weak donor character of strains A53 and A58 became more expressed at 10 −1 MKNO 3 concentration. Te second category is that three strains A30, A60, and A63 expressed a weaker character in a higher ionic strength. For the other strains, no expression of the donor trait was obtained at higher ionic strength. In a suspension with a concentration of 10 −3 KNO 3 , only two strains expressed an electron acceptor character. Tis character is very important for strains A49, A57, A58, A60, A63, and A65 at 10 −1 MKNO 3 . Tis work has shown that these properties vary greatly depending on the Streptomyces strain. It is important to consider the change in physicochemical properties of surface cells with ionic strength when using Streptomyces in diferent bioprocesses. 1.Introduction Streptomyces are Gram•positive flamentous bacteria be• longing to the phylum Actinobacteria [1]. Tey are ubiquitous in a variety of natural and artifcial environ• ments and constitute a large fraction of soil microbial populations [2, 3]. Tese bacteria are characterized by a complex and peculiar developmental cycle [1, 4]. Streptomycetes are extensively studied as producers of a wide variety of natural metabolites of biotechnological interest [5–8]. Tey produce about 75% of commercially and medically useful antibiotics and about 60% of those developed for agriculture [9, 10]. However, few studies have highlighted the interfacial interactions between this bacterium and its environment that could play a critical role in the process of producing bioactive molecules and bioenvironment activities [11, 12]. Van der Waals elec• trostatic and acid•base interactions are associated with bacterial adhesion phenomena depending on the physi• cochemical properties of the substrate and the bacterial surface such as hydrophobicity and electron donor/ac• ceptor properties [13–17]. Hindawi International Journal of Microbiology Volume 2023, Article ID 8841509, 8 pages https://doi.org/10.1155/2023/8841509