Journal of Biotechnology 230 (2016) 1–2 Contents lists available at ScienceDirect Journal of Biotechnology journal homepage: www.elsevier.com/locate/jbiotec Genome announcement Complete genome sequence of a low-temperature active and alkaline-stable endoglucanase-producing Paenibacillus sp. strain IHB B 3084 from the Indian Trans-Himalayas Hena Dhar a,b , Mohit Kumar Swarnkar b , Aditi Rana b , Kanishak Kaushal b , Anil Kumar Singh a,b,1 , Ramesh Chand Kasana b,2 , Arvind Gulati a,b,∗ a Academy of Scientific and Innovative Research, New Delhi, 110 025, India b CSIR—Institute of Himalayan Bioresource Technology, Palampur, HP, 176 061, India a r t i c l e i n f o Article history: Received 8 April 2016 Accepted 19 April 2016 Available online 22 April 2016 Keywords: Paenibacillus Complete genome Endoglucanases Cold adaptation a b s t r a c t A genome of 5.88 Mb with 46.83% G+C content is reported for an endoglucanase-producing bacterium Paenibacillus sp. strain IHB B 3084 isolated from the cold environments of the Indian Trans-Himalayas. The psychrotrophic bacterium produces low-temperature active and alkaline-stable endoglucanases of industrial importance. The genomic data has provided insight into genomic basis of cellulase production and survival of the bacterium in the cold environments. © 2016 Published by Elsevier B.V. Microorganisms from the cold environments are a potential source of industrially important cellulases, proteases, lipases and amylases (Adrio and Demain, 2014). Cold-active and alkaline cel- lulases find application in textile and detergent industries with the advantages of energy saving, compatibility with detergent additives and prevention of wear and tear of textile fibers and backstaining during stonewashing. Paenibacillus sp. strain IHB B 3084 isolated from the lake sediments from Gete (latitude 32 ◦ 18 ′ 20.9 ′′ and longitude 78 ◦ 01 ′ 31.7 ′′ ) of Lahaul-Spiti in the Western Himalayas is a Gram-positive, motile and facultative anaerobic bac- terium. The 16S rRNA gene sequence analysis of the bacterium showed the highest similarity of 98.6% with Paenibacillus terrae AM141 T . The genes encoding low-temperature and mild-alkaline active endoglucanases with different biochemical properties from the bacterium were cloned and expressed in Escherichia coli (Dhar et al., 2015a, 2015b). The genomic DNA of Paenibacillus sp. IHB B 3084 isolated using GenElute TM Bacterial Genomic DNA Kit (Sigma-Aldrich, USA) was ∗ Corresponding author at: CSIR—Institute of Himalayan Bioresource Technology, Palampur 176 061, India. E-mail addresses: gal arvind@yahoo.co.in, arvindgulati@ihbt.res.in (A. Gulati). 1 Present address: ICAR—Indian Institute of Agricultural Biotechnology, PDU Cam- pus, IINRG, Namkum Ranchi, 830 410, India. 2 Present address: ICAR—Central Arid Zone Research Institute, Jodhpur, Rajasthan, 342 003, India. fragmented to 10 kb using Covaris ® g-TUBE ® device (Covaris Inc., USA). The complete genome sequence was obtained by PacBio RS II Single Molecule Real Time (SMRT) sequencing technology (Pacific Biosciences, Menlo Park, CA), employing P6 polymerase and C4 sequencing chemistry with a 240-min movie time on a single SMRT cell (Kiran et al., 2015). De novo assembly of 31,143 reads (N50 size 29,927 and mean read length 15,738) of 490,148,207 nucleotides using hierarchical genome assembly process protocol version 3.0 in SMRT Analysis version 2.2.0 (Pacific Biosciences, USA) generated a complete genome sequence with 75X coverage. The complete genome of Paenibacillus sp. strain IHB B 3084 of 5.88 Mb size with 46.83% G+C content consisted of one chromo- some of 5.5 Mb and six plasmids of 2.9–115.5 kb (Table 1). The annotation of genome sequence on the Rapid Annotations using Subsystems Technology server predicted 6093 protein-coding genes, 119 RNA genes (92 for tRNA and 27 for rRNA) and 451 sub- systems (Aziz et al., 2008). A total of 725 genes were assigned for carbohydrate metabolism, 404 for amino acids and derivatives, and 104 for stress response, including 22 for heat shock, 4 for cold shock and 3 for alkaline shock. The genes predicted for cold shock pro- teins (CspC and CspD), alkaline shock proteins and excinuclease ABC (UvrABC) in the strain IHB B 3084 have been related to stress tolerance in the bacteria from stressful environments (Zhou et al., 2016). In addition, the prediction of genes encoding for endoglu- canases (6), cellobiosidase (1) and -glucosidases (7) corroborated the production of cellulases by the strain. The presence of genes http://dx.doi.org/10.1016/j.jbiotec.2016.04.037 0168-1656/© 2016 Published by Elsevier B.V.