Production of Hydrogen Using Nanocrystalline Protein-Templated Catalysts on M13 Phage Brian Neltner, Brian Peddie, Alex Xu, William Doenlen, Keith Durand, Dong Soo Yun, Scott Speakman, Andrew Peterson, § and Angela Belcher †,, * Department of Materials Science and Engineering, Department of Mechanical Engineering, § Department of Chemical Engineering, and Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 H ydrogen fuel cells generate power by chemically converting hydro- gen and oxygen into water. This process does not produce power through the use of a heat engine, so the maximum efficiency can be quite high. 1 Although hy- drogen has a mass energy density of 141.8 MJ/kg, the volumetric energy density is only 0.0116 MJ/L for uncompressed hydrogen and only 11.4 MJ/L for hydrogen com- pressed to 100 MPa (not including the weight and volume of the tank required to maintain such a high pressure). By compari- son, ethanol has an energy density of 29.7 MJ/kg but has a volumetric energy density of 23.4 MJ/L at atmospheric pressure. 2 By catalytically reforming ethanol into hydro- gen gas, the benefits of having a high en- ergy density fuel can be combined with the benefits of using hydrogen fuel cells to per- form work more efficiently, resulting in a significantly more practical way to incorpo- rate fuel cells into existing systems. The catalytic oxidative steam reforming of ethanol-water mixtures saw a major breakthrough in 2004, when the complete conversion of ethanol over rhodium-ceria (Rh@CeO 2 ) catalysts was observed at 650 °C. 3 Since then, the conversion of ethanol into hydrogen gas over ceria (CeO 2 ) has been widely investigated and makes a good prototypical reaction to study the effects of biotemplating and nanocrystallinity on catalytic performance. 3-24 The overall oxi- dative steam reforming reaction is and is slightly exothermic with H R -50 kJ/mol, allowing for autothermal operation when combined with a limited amount of total combustion. 3 In this reaction, water is consumed, so the molar ratio of H/C in the product stream is not equal to the ratio found in ethanol (3:1). Combining rhodium with nickel on CeO 2 (Rh-Ni@CeO 2 ) enhances the ability of the catalyst to oxidatively steam reform eth- anol, especially at low temperatures. 6,10 The presence of both nickel and rhodium allows for the efficient decomposition of ethanol to smaller molecules through C-C bond cleavage, as well as the conversion of meth- ane, CO, and H 2 O to H 2 and CO 2 . Nickel alone tends to produce more acetaldehyde than the mixed system, and rhodium alone tends to produce large amounts of CO and CH 4 compared to the mixed system. Nickel is highly active in the steam reforming nec- essary to convert methane and methanol into hydrogen and carbon dioxide in the presence of water and also to allow for the water gas-shift reaction to convert residual carbon monoxide and water into hydrogen and carbon dioxide. 6,25 *Address correspondence to belcher@mit.edu. Received for review February 19, 2010 and accepted May 24, 2010. Published online June 7, 2010. 10.1021/nn100346h © 2010 American Chemical Society C 2 H 5 OH + 2H 2 O + 1/2O 2 f 2CO 2 + 5H 2 (1) ABSTRACT For decades, ethanol has been in use as a fuel for the storage of solar energy in an energy-dense, liquid form. Over the past decade, the ability to reform ethanol into hydrogen gas suitable for a fuel cell has drawn interest as a way to increase the efficiency of both vehicles and stand-alone power generators. Here we report the use of extremely small nanocrystalline materials to enhance the performance of 1% Rh/10% Ni@CeO 2 catalysts in the oxidative steam reforming of ethanol with a ratio of 1.7:1:10:11 (air/EtOH/water/argon) into hydrogen gas, achieving 100% conversion of ethanol at only 300 °C with 60% H 2 in the product stream and less than 0.5% CO. Additionally, nanocrystalline 10% Ni@CeO 2 was shown to achieve 100% conversion of ethanol at 400 °C with 73% H 2 , 2% CO, and 2% CH 4 in the product stream. Finally, we demonstrate the use of biological templating on M13 to improve the resistance of this catalyst to deactivation over 52 h tests at high flow rates (120 000 h 1 GHSV) at 450 °C. This study suggests that the use of highly nanocrystalline, biotemplated catalysts to improve activity and stability is a promising route to significant gains over traditional catalyst manufacture methods. KEYWORDS: ceria · catalyst · ethanol · fuel cell · hydrogen ARTICLE www.acsnano.org VOL. 4 NO. 6 3227–3235 2010 3227