Current Pharma Research ISSN: 2230-7842 CPR 2(3), 2012, 571-579. 571 Evaluation of Anti Epileptic Activity of Leaf Extract of Cynodon Dactylon (L.) Pers. in Validated Animal Models. * 1 G.Venkateswarlu, 1 K.Edukondalu, 2 B.G.V.Chennalakshmi, 1 P.Sambasivarao, 1 G.Raveendra, 3 V.Ramanarayana Reddy. 1 Department of Pharmacy, DCRM Pharmacy college, Prakasam (Dt), Andhra Pradesh, India, 2 Department of Pharmacy, Vasavi College of Pharmacy, Tadepallygoodem, Andhra Pradesh, India, 3 Department of Pharmacy practice, KLR Pharmacy College, Paloncha, Andhra Pradesh, India. Abstract The main objective of the work is to evaluate the antiepileptic activity and to evaluate antiepileptic activity of alcoholic and aqueous extracts of leaf extracts of Cynodon dactylon in validated animal models. For assessing of anticonvulsant activity Pentylenetetrazole (PTZ), Maximal electro shock (MES), Strychnine and Picrotoxin induced convulsive models were used. Preliminary Phytochemical investigation of the Ethanolic extract of Cynodon dactlon (EECD) leaves reveals the presence of carbohydrates, Amino acids, Flavonoids, Tannins, Fixed oils, Fats, Glycosides, and saponins. However, the qualitative test for Proteins and Alkaloids yielded negative results. In PTZ, Strychnine and Picrotoxin induced convulsion models, medium and high doses (600 and 800mg/kg respectively), but not the low dose (400mg/kg) of EECD showed significant anti-convulsant activity by delaying the onset of convulsions and by prolong the onset of clonus and tonic-extensor convulsion. In MES induced convulsion model medium and high doses (600 and 800mg/kg respectively) but not the low dose (400mg/kg) of EECD had exhibited significant anticonvulsant effect by decreasing the duration of tonic- extensor phase and prolonging the onset of clonus convulsion. Concluded that the all models, convulsions induced by either chemicals or MES, the EECD exhibited a fairly good anticonvulsant effect. Key Words Cynodon dactylon, anticonvulsant and antiepileptic. Introduction An ideal antiepileptic drug should suppress all seizures without causing any unwanted effect. Unfortunately, the drugs available in the modern medicine not only fail to control the seizure activity in some patients, but quite frequently cause unwanted effects that range in severity from minimal impairment of the CNS to death from aplastic anemia or hepatic failure. 1 Rich floral biodiversity of India has provided herbal health practitioners and other traditional healers in the country with an impressive pool of „natural pharmacy‟ from which plants are selected as ingredients to prepare herbal remedies and medicines (phytomedicine) for the treatment 2 , management and control of a variety of human ailments. The plant posses anti microbial 3 and anti viral activity and has also been used to treat urinary tract infection, calculi and prostatitis. The plant aqueous extract is used as anti inflammatory, *Corresponding Author: venkey22pharma@gmail.com diuretic, anti emetic and purifying agent. Leaves have been used as anti epileptic agent in traditional system of medicine in India 4 . But, its antiepileptic activity is not yet validated scientifically as on date. Hence in the current dissertation the antiepileptic activity of leaf extract of Cynodon dactylon in validated animal models is considered 5 . Materials and Methods Materials Diazepam was a Gift sample from Ranbaxy Laboratories Ltd, Mumbai, India. Pentylenetetrazole was a Gift sample from Sigma-Aldrich, St.Louis, MO 63103 USA. Strychnine was a Gift sample from Sigma-Aldrich, St.Louis, MO 63103 USA. Picrotoxin was a Gift sample from Sigma-Aldrich, St.Louis, MO 63103 USA. Tween-80 was a Gift sample from S.D.fine Chem Ltd. Mumbai. All other chemicals and ingredients were used for study are of Analytical grade.