Volume 8, Issue 8, August – 2023 International Journal of Innovative Science and Research Technology ISSN No:-2456-2165 IJISRT23AUG1033 www.ijisrt.com 623 Formulation and Development of Liquid Herbal Syrup by using Guava, Indian Mulberry, Pomegranate and Apple Fruit Extract Shibanjan Paul Roy, Kamal Deka, Shyam Prakash Rai Abstract:- Our research about formulation and proper development of a liquid herbal based syrup by using a proper combination of guava fruit, Indian mulberry fruit, pomegranate fruit and apple fruit extracts is described in our research study. Our research objective was to create a natural herbal syrup that properly harnesses the potential health benefits by these fruits extracts. Our research involved by selecting the fruits extracts based on their individual medicinal properties and synergistic effects. Our research methods included fruit extraction, formulation and Development with sensory evaluation. Our results indicated a very successful development of liquid herbal based syrup, by providing a good and potential natural remedy with various health benefits. This research is guided and written skills done by Guide cum Research-Teacher-in-Charge Mr. Shibanjan Paul Roy, Mr. Kamal Deka and Mr. Shyam Prakash Rai performed for the practical and others work and note the reading under his guidance and observation. I. INTRODUCTION The herbal preparations have been properly utilized for centuries to address properly for various health conditions and promote well being. The fruits like Guava, Indian mulberry, pomegranate and apple fruits are renowned for their nutritional and medicinal values. Guava is very rich in vitamin C and has antioxidant activity otherside Indian mulberry is a good source of vitamins and minerals, pomegranate fruit contains polyphenols compounds and has antiinflammatory activity, while apples are well packed with dietary fiber and various essential nutrients. In this research by combining these fruits into a liquid herbal syrup offered a synergistic effect by enhancing their health benefits. Our research study was to develop a liquid herbal syrup that incorporates the goodness of these type fruits extracts to offer a natural and effective remedy. Our formulation properly process was properly designed to preserve the active compounds present in the fruits while we ensuring an appealing taste for easy consumption, especially for those matter who prefer natural alternatives to conventional medications. II. MATERIALS WITH PROPER METHODS In our research our herbal based syrup was prepared. we first collected the fruits extracts from local market near our lab. After the fruits were properly peeled for obtain about the edible portion.250.001gm of this edible portions were chopped in a blender. After we used the whatsman filter paper for the fresh extracts. After our fresh extracts used and making the F1 and F2. III. ANALYSIS AND ISPECTION OF ANTIBACTERIAL ACTIVITY In this research we used agar well diffusion method to determine properly of antibacterial activity of F1 and F2 aganist pathogens of S. aureus and S. mutans. As in microbial suspension the secondary cultures were dispersed as evenly on surface of the rose bengal and mullen hinton agar plates by using spreader with sterile medium. Different concentrations of F1 and F2 of 25μl,50μl and 100μl as incorporated through micropipette into wells as created on agar plate by using sterile cork borer. After we incubated the plates 37°C for 26hr to 46hr.Commercial antibiotic Amoxycillin 50mg/ml we used as positive control for S.aureus and S.mutans. As we recorded the zone of inhibition for each of the plate and compared as the control group. all of our tests were properly replicated as triplicate for inspection and analysis. As 35ml of syrup of F1 we did as 24.6ml of invert sugar base +sweet lemon oil 3.5ml+ mixed guava fruit extract 2.4ml,pomegranate fruit 2.4ml,indian mulberry 1.925ml and apple fruit 0.175ml of F1 and F2 Table 1 Formulation F1 of 35ml of syrup Ingredients Quantity Guava fruit extract 2.4ml Pomegranate fruit extract 2.4ml Indian mulberry fruit extract 1.925ml Apple fruit extract 0.175ml Sweet lemon oil 3.5ml Invert sugar base 24.6ml