SUPPLEMENTARY MATERIAL Cell death induction by mycelium extracts from Pleurotus spp. on cervical cancer cell lines Carla O. Contreras-Ochoa a , Jocelyn Maza-Lopez b , Pedro Mendoza de Gives b , Liliana Aguilar- Marcelino b , Carlos Mojica-Cardoso c , Jisela Dimas-González d , Diana L. Fernández-Coto a , Jesús Reyna-Figueroa e , Ma. Eugenia López-Arellano b * and Alfredo Lagunas-Martínez a *. a Centro de Investigación sobre Enfermedades Infecciosas. Instituto Nacional de Salud Pública. b Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. c Laboratorio de Patología, Hospital del Niño Morelense. d Centro Nacional de la Transfusión Sanguínea. e Departamento de Enseñanza e Investigación, Hospital Central Sur de Alta Especialidad Petróleos Mexicanos. Abstract Mushrooms have health benefits, including anti-tumoral properties. We evaluated the cytotoxic and cell death induction effects of water-soluble extracts of Pleurotus ostreatus and Pleurotus eryngii mycelium in the cervical cancer cell lines HeLa (HVP18 + ) and SiHa (HVP16 + ) as well as the non-tumoral cell line HaCaT. Both Pleurotus extracts presented similar protein patterns from 190 to 10 kDa and displayed protease activity on a gelatine substrate. The mycelium extracts of both Pleurotus strains induced a dose-dependent cytotoxic effect on HPV + cells (IC 50 65 μg) , whereas HaCaT cells were less susceptible (IC 50 90 μg) . The cytotoxic effect at the IC 50 concentration was not associated with apoptosis, the activation of Caspases-3/7 was not significantive; only P. eryngii induced a moderate (1.2-fold) increase in SiHa cells. Pleurotus extracts induced autophagy, mainly in SiHa cells (4.3-fold) . Neither extracts induced changes in p53 protein expression, suggesting that the cytotoxic effect could be due to p53-independent pathways.