This article is protected by copyright. All rights reserved Cellular and Metabolic Engineering Biotechnology and Bioengineering DOI 10.1002/bit.26188 FX Knockout CHO Hosts can Express Desired Ratios of Fucosylated or Afucosylated Antibodies with High Titers and Comparable Product Quality † Short Running Title: “Fucosylated and Afucosylated Antibodies in One Host”. Salina Louie 1 , Benjamin Haley 2 , Brett Marshall 4 , Amy Heidersbach 2 , Mandy Yim 1 , Martina Brozynski 4 , Danming Tang 1 , Cynthia Lam 1 , Bronislawa Petryniak 3 , David Shaw 1 , Jeongsup Shim 4 , Aaron Miller 4 , John B. Lowe 3 , Brad Snedecor 1 , Shahram Misaghi 1* 1 Early Stage Cell Culture, 2 Molecular Biology, 3 Pathology, and 4 Biological Technologies Departments, Genentech, Inc, 1 DNA Way, South San Francisco, California 94080. *Correspondence to: Shahram Misaghi Telephone: 650-467-3685 Fax: 650-225-2006 E-mail: smisaghi@gene.com † This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: [10.1002/bit.26188] Additional Supporting Information may be found in the online version of this article. This article is protected by copyright. All rights reserved Received May 20, 2016; Revision Received September 9, 2016; Accepted September 23, 2016 Accepted Preprint