Nova Biotechnol Chim (2023) 22(1): e1414 DOI: 10.34135/nbc.1414 1 Nova Biotechnologica et Chimica Effects of dihydroquercetin, 1-aryltetrahydroisoquinoline, and conjugate on the functional condition mitochondrial membrane of the rat liver Zafarjon M. Ernazarov 1, , Mamurjon K. Pozilov 2 , Muzaffar I. Asrarov 1 , Sherzod N. Zhurakulov 2,3 1 Institute of Biophysics and Biochemistry at the National University of Uzbekistan named after Mirzo Ulugbek, Almazar district, Student town, 174, Tashkent 100174, Uzbekistan 2 National University of Uzbekistan named after Mirzo Ulugbek, University str. 4, Tashkent 100174, Uzbekistan 3 Acad. S. Yunusov Institute of the Chemistry of Plant Substances Academy of Sciences of Uzbekistan, M. Ulugbek st. 77, Tashkent 100170, Uzbekistan  Corresponding author: zafarbek1985@gmail.com Article info Article history: Received: 14 th October 2021 Accepted: 20 th October 2022 Keywords: DHQ-11 conjugate F-18 isoquinoline alkaloid Fe 2+ /citrate Lipid peroxidation Mitochondria Abstract In the current research paper, the flavonoid dihydroquercetin, 1-(2´-bromine-4´,5´- dimethoxyphenyl)-6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline (F-18) and 2-(3,4- dihydroxyphenyl)-6-(1-(2´-bromine-4´, 5´-dimethoxyphenyl)-6,7-dimethoxy-3,4- dihydroisoquinoline-2 (1N)-il) methyl-3. The effects of 5,7-trigidroxychroman-4-on (DHQ-11) conjugate on rat liver mitochondrial calcium megachannels and on lipid peroxidation (LPO) induced using Fe 2+ /citrate were investigated in vitro experiments. White male rats weighing 180-200 grams were used in the experiments. It was found that the DHQ-11 conjugate was identified to have an inhibitory effect on rat liver mitochondria to calcium megachannels and peroxidation of lipids induced by Fe 2+ /citrate. The inhibitory properties of DHQ-11 conjugate on hepatic mitochondrial calcium megachannels and mitochondrial membrane lipid peroxidation were identified as active against dihydroquercetin and the F-18 isoquinoline alkaloid. © University of SS. Cyril and Methodius in Trnava Introduction These days, biologically active substances that are being extracted from plants are widely utilised in medicine for the purpose of treatment and prevention of diseases. This is because substances that are derived from plants are distinguished from synthetic drugs by their non-toxic effects on cells and their high biological activity in small concentrations. Cell mitochondria serve as molecular targets for a number of biologically active compounds. Being located in the outer and inner membrane of the mitochondria, the number of ion transport channels modulated by specific activators and inhibitors (Drahota et al. 2009). The calcium megachannel (mitochondrial permeability transition pore-mPTP) can modify its physiological conformation due to increased loading of Ca 2+ ions in the matrix (Paul et al. 2008). Currently, there are many tools that could increase mPTP permeabilization. However, pharmaceuticals that inhibit mPTP permeability are encountered quite