Supplementary Information An engineered nanoplatform for bimodal anticancer phototherapy with dual- color fluorescence detection of sensitizers Aurore Fraix, a Noufal Kandoth, a Ilse Manet,* b Venera Cardile, c Adriana C. E. Graziano, c Ruxandra Gref d and Salvatore Sortino* a a Laboratory of Photochemistry, Department of Drug Sciences, University of Catania, I-95125 Catania, Italy. E-mail: ssortino@unict.it b Istituto per la Sintesi Organica e la Fotoreattività-CNR, I-40129, Bologna Italy. E-mail: ilse.manet@isof.cnr.it c Department of Bio-Medical Sciences, Physiology Division, University of Catania, I-95125 Catania, Italy. d UMR CNRS 8612, Faculty of Pharmacy, Paris Sud University, 92290 Châtenay-Malabry, France. ________________________________________________________________________________ S1. Chemicals. All chemicals were purchased by Sigma-Aldrich and used as received. Compound 2 was purchased from Frontier Scientific and used as received. All solvents used were spectrophotometric grade. S2. Instrumentation. NMR spectra ( 1 H NMR recorded at 500 MHz, 13 C NMR recorded at 125 MHz) were obtained on Varian Instruments and are referenced in ppm relative to TMS or the solvent signal. UV/vis absorption spectra were recorded with a Jasco V 560 spectrophotometer. Fluorescence emission spectra were recorded with a Spex Fluorolog-2 (mod. F-111) spectrofluorimeter. Nanoparticle sizes were measured by a dynamic light scattering using a Horiba LS 550 apparatus equipped with a diode laser with a wavelength of 650 nm. Fluorescence quantum yields of 2 and 3 under our experimental conditions were determined by using 2 in DMF and rodamine B in ethanol as standards, respectively. Fluorescence lifetimes were measured in air-equilibrated solutions with a time correlated single photon counting system (IBH Consultants Ltd.). A pulsed laser source at 407 nm (Hamamatsu, 306 mW) operating at 1 MHz frequency was used for excitation and the emission was collected at right angle at 528 or 690 nm. TCSPC was set to 0.0063 ns/channel. The software package for the analysis of the emission decays was provided by IBH Consultants Ltd. Decay profiles were fitted using a multiexponential function and deconvolution of the instrumental response. I(t) =  i a i exp(-t/ i ) (1) f i = (a i  i )/  j (a j  j ) (2) Laser flash photolysis. All of the samples were excited with the second harmonic of a Nd–YAG Continuum Surelite II–10 laser (532 nm, 6 ns FWHM), using quartz cells with a path length of 1.0 cm. The excited solutions were analyzed with a Luzchem Research mLFP–111 apparatus with an orthogonal pump/ probe configuration. The probe source was a ceramic xenon lamp coupled to quartz fiber-optic cables. The laser pulse and the mLFP–111 system were synchronized by a Tektronix TDS 3032 digitizer, operating in pre-trigger mode. The signals from a compact Hamamatsu photomultiplier were initially captured by the digitizer and then transferred to a Electronic Supplementary Material (ESI) for Chemical Communications This journal is © The Royal Society of Chemistry 2013