Production of antimicrobial textiles by cotton fabric functionalization and pectinolytic enzyme immobilization Michaela Coradi a , Micheli Zanetti a, b , Alexsandra Val  erio a ,D  ebora de Oliveira a, * , Adriano da Silva a , Selene Maria de Arruda Guelli Ulson de Souza a , Ant ^ onio Augusto Ulson de Souza a a Department of Chemical and Food Engineering, Federal University of Santa Catarina, Florian opolis, SC, 88040-900, Brazil b Area of Exact and Environmental Sciences, Universidade Comunit aria da Regi~ ao de Chapec o, Chapec o, SC, 89809-000, Brazil highlights graphical abstract  A novel antimicrobial textile was synthesized.  Termamyl ® 2X and Bioprep ® 3000L were investigated for antimicrobial activities.  The periodate precursors have been coated to cotton fabrics to enzyme immobilization.  Coated fabrics showed antimicrobial properties against S. aureus, S. epidermidis, E. coli and C. albicans. article info Article history: Keywords: Antimicrobial textiles Antimicrobial activity Cotton. functionalization Pectinolytic enzyme Immobilization abstract In this work, Termamyl ® 2X and Bioprep ® 3000L were characterized and investigated for their antimi- crobial activities by agar diffusion and Minimum Inhibitory Concentration (MIC). Bioprep ® 3000L enzyme showed higher antimicrobial activity compared to the Termamyl ® 2X against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli and Candida albicans, and was partially active for Pseudomonas aeruginosa, showing a high potential of application to antimicrobial textiles production replacing the synthetic compounds. In addition, Bioprep ® 3000L enzyme was immobilized by covalent bond on the chemically modified cotton fabric surface via periodate reaction (20% immobilization yield) and its antimicrobial activity was investigated. © 2018 Elsevier B.V. All rights reserved. 1. Introduction Natural fibers can be used as materials for finishing and insu- lation in the automotive, construction industry, and particularly suitable for sports textiles production, non-implantable medical products, hygiene and health care products, underwear, shoe lin- ings and packaging industry [1e4]. However, natural fiber fabrics are more susceptible to microorganism attack than synthetic fiber fabrics due to the hydrophilic porous structure that retains water, oxygen and nutrients, providing an ideal medium for the micro- organism proliferation [5]. Currently, many antimicrobial technologies are available in the textile industry, being used in the most different applications to prevent the growth of microorganisms. Due to the biological ac- tivity of the antimicrobial compounds, the safety evaluation of these substances is a subject of constant research. Antimicrobial compounds applied in textile materials should present low toxicity to consumers, be effective against a large spectrum of * Corresponding author. E-mail address: debora.oliveira@ufsc.br (D. de Oliveira). Contents lists available at ScienceDirect Materials Chemistry and Physics journal homepage: www.elsevier.com/locate/matchemphys https://doi.org/10.1016/j.matchemphys.2018.01.019 0254-0584/© 2018 Elsevier B.V. All rights reserved. Materials Chemistry and Physics 208 (2018) 28e34