JAPSC Journal of Animal and Poultry Sciences, 2014, 3 (1): 08-18 Available online at http:/ / www.JAPSC.com Achievement to the goat's spermatogonial stem cells from embryos Alireza Hasani Bafarani 1* , Ziaoddin Mirhoseini 1 , Farid Heidari 2 1 Department of animal science, University of Guilan, Rasht, Iran 2 National Institute of Genetic Engineering and Biotechnology, Tehran, Iran Abstract The present study reports an easy approach to obtain an abundant origin of pluripotent spermatogonial stem cell (SSC) from the goat fetal testis tissue. These cells showed tissue non-specific alkaline phosphatase activity and also express three markers of pluripotency (NANOG, SOX2 and OCT4) which are also expressed in embryonic stem cells. We found that these cells can be used for co-culturing with the inactivated goat embryonic fibroblast for several passages without differentiation or changing in shape. It was also found out that, these cells can remain viable at -70 °C one month by using DMSO. Key Words: Fetal spermatogonial stem cells, Germline stem cells, Goat embryo *Corresponding author: Tel: +989198888162; Fax: +982166940764 E-mail address: ar.hasani@gmail.com