Received 12/13/95; revised 1/13/97; accepted 2/17/97.
The costs of publication of this article were defrayed in part by the payment of
page charges. This article must therefore be hereby marked ads’ertisernent in
accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
I This project was supported by NIH Grant P01-CA41 108. The views expressed
here represent the views of the authors and not those of NIH.
2 To whom requests for reprints should be addressed, at Arizona Prevention
Center, University of Arizona, Tucson, AZ 85724-5126. Phone: (520) 626-7863;
Fax: (520) 321-7754.
3 The abbreviations used are: FFQ, food frequency questionnaire; AFFQ, Arizona
FFQ; PPV, positive predictive value; NPV, negative predictive value; USDA,
United States Department of Agriculture; NFCS, Nationwide Food Consumption
Survey; WBFT, Wheat Bran Fiber Trial; DR. diet record; 4DFR, 4-day food
record.
Vol. 6, 347-354, May 1997 Cancer Epidemiology, Biomarkers & Prevention 347
Use of a Food Frequency Questionnaire to Screen for Dietary Eligibility
in a Randomized Cancer Prevention Phase III ri1
Cheryl Ritenbaugh,2 Mikel Aickin, Douglas Taren,
Nicolette Teufel, Ellen Graver, Kathleen Woolf,
and David S. Alberts
Department of Family and Community Medicine [C. R., M. A., D. T., N. T.,
E. G., K. W.] and Department of Medicine [D. S. A.], The Arizona Cancer
Center, The University of Arizona, Tucson, Arizona 85724
Abstract
Cancer prevention clinical trials use food frequency
questionnaires (FFQs) to assist in eligibility screening.
FFQ reliability and validity studies are available, but
these studies do not evaluate FFQs as screening tools.
The Wheat Bran Fiber Trial of the University of Arizona
used a FFQ as an eligibility screen with the goal of
screening out subjects whose true daily calcium intake
was less than 500 mg per day (for safety) and whose true
dietary fiber intake was greater than 30 g per day (for
safety and trial efficiency). Subjects ineligible by FFQ
were interviewed for final dietary eligibility
determinations. A study was undertaken within the
Wheat Bran Fiber Trial to evaluate the sensitivity,
specificity, positive predictive value, and negative
predictive value (NPV) of the FFQ used in this context.
Four-day food records were collected on 183 potential
participants before entry into the study. Using the 4-day
averages as the “true” value, sensitivity, specificity,
positive predictive value, and NPV were calculated for
men and women separately under two screening
conditions: using the target calcium and dietary fiber
values and using “revised” values identified in interim
analysis within the study.
NPV was acceptable in all analyses. Sensitivity for
low calcium intake was inadequate under the original
criteria (0.33 for men and 0.09 for women) but acceptable
under the revised criteria (0.80 for men and 0.81 for
women). With the revised criteria, specificity declined,
resulting in heavy screening burdens deemed worthwhile
for the safety considerations. Dietary fiber eligibility
screening worked well at target values. These differences
were not predicted by reliability/validity studies.
Introduction
Randomized controlled trials are needed to determine the long-
term effects of diet in the etiology and prevention of cancer.
Ideally, such trials recruit panicipants who are not already on
the intervention and randomize them to experimental or control
conditions. FFQs3 are currently in use as screening tools to
identify suitable participants in the Colon Cancer Prevention
Program Project at The University of Arizona, and in the
Women’s Health Initiative, a NIH-funded nationwide trial (1).
The FFQ method has been applied in epidemiological research
to the etiology of cancer and cardiovascular diseases, in which
one or more dietary components is either the factor of interest
or a confounding variable. FFQs have been subjected to reli-
ability and validity analysis for use in these epidemiological
studies. However, use in eligibility screening requires informa-
tion on the sensitivity and specificity characteristics of the
instrument, analyses that have not been widespread to date.
Multiple DRs have generally been considered to yield the
most valid estimates of individuals’ usual intakes but they are
far from perfect (a flawed “gold standard”). In the United
States, recent studies comparing energy estimates from DRs
with energy estimates from doubly labeled water find an aver-
age underestimate of about 200 kcal/day, although this varies
with the population studied (2-4). However, even the use of
DRs is in many study settings limited by the time and expense
they require. The quantitative FFQs are designed to measure
average long-term diet or usual consumption of specified food
items rather than to provide a precise estimate of short-term
intake. The low cost and ease of administration of FFQs facil-
itates their use for screening in large clinical trials; they impose
the least respondent burden of all dietary assessment methods in
common use and, if accompanied by clear instructions, can be
self-administered successfully in many populations.
Assessment of the quality of a measurement entails con-
sideration of its reliability and of the validity of the technique
used to derive the measurement. Reliability may be defined as
the level of agreement between replicate measurements under
the same conditions (repeatability), and validity may be defined
as the extent to which a measurement technique measures that
which it aims to measure (accuracy). These two aspects of
quality are related in that a valid measurement technique gives
measurements that are repeatable. A measure that is not repeat-
able may lead to misclassification of individuals upon catego-
rization with respect to that measurement. Dietary assessment
methods usually contain a high degree of random misclassifi-
cation due to random measurement error. Random measure-
ment error often attenuates risk estimates of the diet-disease
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