_____________________________________________________________________________________________________ *Corresponding author: E-mail: nikigargi.15@gmail.com, niki.gargi15@gmail.com; Int. J. Environ. Clim. Change, vol. 12, no. 12, pp. 1939-1954, 2022 International Journal of Environment and Climate Change Volume 12, Issue 12, Page 1939-1954, 2022; Article no.IJECC.101867 ISSN: 2581-8627 (Past name: British Journal of Environment & Climate Change, Past ISSN: 2231–4784) Seed Germination and Seedling Vigour of Spine Gourd (Momordica dioica Roxb.) in Response to Different Physical and Chemical Treatments Gargi Gautami Padhiary a* , Gouri Shankar Sahu a , Pradyumna Tripathy a , Gyana Ranjan Rout b and Kailash Chandra Samal c a Department of Vegetable Science, College of Agriculture, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha, India. b Department of Molecular Biology and Biotechnology, College of Agriculture, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha, India. c Department of Molecular Biology and Biotechnology and Plant Physiology, College of Agriculture, Odisha University of Agriculture and Technology, Bhubaneswar, Odisha, India. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/IJECC/2022/v12i122214 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/101867 Received: 25/10/2022 Accepted: 28/12/2022 Published: 31/12/2022 ABSTRACT The present investigation was carried out at Department of Vegetable Science, OUAT, Bhubaneswar during 2019-2021. Five to six months old stored spine gourd seeds were exposed to various physical (T 1 : control, T 2 : 48 hours soaking in water, T 3 : hot water treatment for 20 minutes + 48 hourssoaking in water, T 4 : scarification by sand paper + 48 hours soaking in water, T 5 : removal Original Research Article