Pramita Wally, Andi Sitti Marwah & Azril Fajar Warang 224 Jurnal Biotek p-ISSN: 2581-1827 (print), e-ISSN: 2354-9106 (online) Website: http://journal.uin-alauddin.ac.id/index.php/biotek/index Efektivitas Ekstrak Myristica fragrans Houtt Terhadap Bakteri Patogen Pseudomonas aeruginosa dan Methicilin Resistensi Staphylococcus aureus Pramita Wally 1* , Andi Sitti Marwah 1 , Azril Fajar Warang 1 1 Universitas Muhammadiyah Maluku, Indonesia *Correspondence email: pramitawally3@gmail.com (Submitted: 22-09-2022, Revised: 30-11-2022, Accepted: 17-12-2022) ABSTRAK Pala (Myristica fragrans Houtt), diperkaya dengan metabolit sekunder dan telah dipercayai secara tradisional bermanfaat dalam pengobatan infeksi dan inflamasi. Tujuan penelitian ini adalah untuk mengetahui kosentrasi ekstrak pala yang efektif dalam penghambatan Pseudomonas aeruginosa dan Methicillin Resistant Staphylococcus aureus (MRSA) sebagai bakteri patogen kulit. Jenis penelitian adalah eksperimen laboratorium untuk mengukur aktivitas antibakteri secara in-vitro melalui teknik disk diffusion Kirby and Bauer. Prosedur kerja meliputi uji fitokimia, ekstraksi serta uji aktivitas antibakteri. Hasil penelitian berdasarkan uji one way Anova menunjukkan bahwa f-hitung ˃ f-tabel yang berarti bahwa ekstrak daging buah pala efektif dalam menghambat P. aeruginosa dan Meticilin Resistensi Staphylococcus aureus secara keseluruhan total perlakuan. Konsentrasi 80% merupakan konsentrasi tertinggi dengan rerata diameter zona hambat terhadap bakteri P. aeruginosa 17.3 mm sementara 19,6 mm untuk zona hambat Methicilin Resistensi Staphilococcus aureus. Nilai rerata diameter zona hambat tersebut dikategorikan kuat sebagai zat antibakteri. Kata Kunci: antimikroba, MRSA, pala (Myristica fragrans Houtt), patogen, P. aeruginosa ABSTRACT Nutmeg (Myristica fragrans Houtt) is enriched with secondary metabolites and has been traditionally believed to be useful in the treatment of infection and inflammation. The aim of this study was to determine the effective concentration of nutmeg extract in inhibiting skin pathogenic bacteria Pseudomonas aeruginosa and Methicillin-Resistant Staphylococcus aureus (MRSA). The research type was a laboratory experiment to measure antibacterial activity in vitro through Kirby and Bauer's disk diffusion technique. Work procedures included a phytochemical test, extraction, and antibacterial activity tests. The results of the study based on the one-way ANOVA test showed that f-count ˃ f-table which means that nutmeg pulp extract was effective in inhibiting P. aeruginosa and Meticillin Resistance of Staphylococcus aureus as a whole total treatment. The concentration of 80% was the highest concentration, with an average diameter of the inhibition zone against P. aeruginosa of 17.3 mm and 19.6 mm for the inhibition zone of Meticillin-Resistant Staphylococcus aureus. The mean value of the diameter of the inhibition zone is categorized as a strong antibacterial substance. Keywords: antimicrobial, MRSA, nutmeg (Myristica fragrans Houtt), pathogens, P. aeruginosa Copyright©2022