Fairoz Basha and Rao Biological Forum – An International Journal 15(3): 696-712(2023) 696 ISSN No. (Print): 0975-1130 ISSN No. (Online): 2249-3239 Taxonomy Studies: An Overview on Isolation and characterization of Available Resources of Marine Microalgae for Bio-fuel Production Fairoz Basha M. 1 * and Shrikanya Rao K.V.L. 2 1 Ph.D. Scholar, Department of Biotechnology, Vikrama Simhapuri University, Kakutur, Nellore (Andhra Pradesh) India. 2 Assistant Professor, Department of Biotechnology, Vikrama Simhapuri University, Kakutur, Nellore (Andhra Pradesh) India. (Corresponding author: Fairoz Basha M.*) (Received: 19 January 2023; Revised: 24 February 2023; Accepted: 27 February 2023; Published: 22 March 2023) (Published by Research Trend) ABSTRACT: Microalgae have been observed as a viable biomass for the manufacture of biofuels, but the scaled-up culture of these organisms has challenges regarding freshwater resource use. To overcome the restrictions of water resources, it is essential to obtain robust marine strains that can produce triacylglycerols and high-value-added metabolites. The present study was conducted to identify microalgae from the Indian Sea and its environs which included toxified sites, tenable for biofuel production. 4 stations belong to the Nellore district (S-1 Mypadu, S-2 Kothakoduru, S-3 Nelatru, S4- Krishna Patnam) with this district is special for aquaculture industries like shrimp and fish culture also more hatcheries and processing, and Nellore beaches located in the east coast of Bay of Bengal of India. Sixty-four (64) species of microalgae were acquired in 4 terminals. On further isolation, 19 species with a process-oriented pressure choice were developed through artificial insemination cultures. This basic research provides an overview of the isolation and characterization of available resources of marine microalgae for biofuel production. Keywords: Marine Microalgae, Isolation, Taxonomy, Diatom, Bio-fuel production. INTRODUCTION Microalgae offer a feasible biomass feedstock to manufacture ethanol as the demand for biofuels rises internationally (Culaba et al., 2020). One of the different feed stocks for biofuel production, microalgae have obtained great interest over the last few years being obligated to pay to their possible higher efficiencies, high materials of triacylglycerols as well as adaptability for growing on the non-arable structure. Having pointed out that, some vital specific problems need to have to must become addressed right before algal biofuel ends up being a company reality (Illman et al., 2010). As instance, considerable centered obstacles continue to live in the source of unique algae stress, greater group functionality, in addition to reduced mowing price (Schenk et al., 2009). On top of that, commercialization of algal biofuels is based upon restrictions of water sources and also furthermore company economics. As there are distinguishing uses for readily available freshwater, whereas saltwater is bountiful, it is a lot more practical to make use of microalgae adjusted to saltwater for biofuel production. None of the hit-and-miss campaigns today has possessed the ability to show efficient production of biofuel stemming from microalgae. Because of that, tensions with essential co-products make use of a method for expense decline (Lee and Lee 2001). New methods such as hybrid indoor helical-tubular photo bioreactor was just recently proposed for growing and cultivating microalgae (Hashemi et al., 2020). Emerging technologies such as hydrothermal liquification methods are also employed to develop the biofuel microalge (Devi and Parthiban 2020). Since lot of bioactive items have been covered as possessing the potential to be practical co-products, such as pigments, antioxidants, eicosapentaenoic acid, docosahexaenoic acid, as well as biofertilizers (Radmann and Costa 2008). One of them, astaxanthin is an effective, high- value, organic antioxidant. Astaxanthin production alongside microalgae has achieved substantial commercial excellence. On top of that, picking up microalgae is one of more primary problems in the significant production of microalgae-derived biofuels as a result of the size of their small cells along with also minimized focus in cultures. It was predicted that the fee for accumulating algal biomass represents 20%- 30% of the comprehensive production expenditures. Therefore, a worry alongside the parts of having greater biomass performance, greater body system fat deposits product, helpful co-products, efficient- harvesting Biological Forum – An International Journal 15(3): 696-712(2023)