Journal of Experimental Sciences 2011, 2(8): 10-13 ISSN: 2218-1768 www.scholarjournals.org www.jexpsciences.com 10 JES-Physical Sciences Spectroscopy Studies on the Status of Aloin in Aloe vera and Commercial Samples S. Ravi 1* , P. Kabilar 2 , S. Velmurugan 1 , R. Ashok Kumar 1 and M. Gayathiri 3 1 Department of Engg. & Physics, 2 Department of DDE Wing, 3 Department of Horticulture, Annamalai University, Annamalai Nagar – 608 002, Tamil Nadu, India Article Info Abstract Article History This study was carried out at Department of Physics and Department of Horticulture, Annamalai University, Annamalai Nagar, Tamil Nadu, India to identity and characterize the phenolic antroquitones (Aloin-A and B) from Aloe Vera samples. Among the lewenth different samples forms of Aloe vera. The Aloe vera sap contain more aloin of 4 hydroxy aloin. Aloe vera leaf, gel,root commercial gel and commercial soap samples were characterized by FT-IR and UV Spectroscopy techniques. The result were discussed the above studies. Received : 05-05-2011 Revised : 11-07-2011 Accepted : 12-07-2011 *Corresponding Author Tel : +91-9698803130 Email: ambedravi@yahoo.com ©ScholarJournals, SSR Key Words: Aloe vera, Aloin-A and Aloin-B, FT-IR(Fourier Transform Infrared Spectroscopy), UV(Ultra Violet Spectroscopy) techniques Introduction Aloe vera L. (Aloe barbadensis miller) is an important medicinal plant belongs to the family Liliaceae. It has larger demands and is traded in medicinal drug markets of the world for flavouring liquid and a source of ‘aloin’ (4.5 to 25 per cent). In recent times, herbal remedies are gaining their prominence, because of the observation that the efficacy of allopathic medicines such as antibiotics which once had near universal objectiveness against serious infections is on the wane. Over the years, infectious agents have developed resistance to synthetic drugs and the herbs and their active constituents are now being increasingly used to treat various diseases. The ability of herbal medicine to object body systems depends on the chemical constituents that it contains. Aloe products have long been used in health foods and for medical and cosmetic purposes. These products range from aloe drink to aloe gels, powders, capsules, creams etc. for both internal and external uses for a wide variety of indications. Aloe has a wide range of medicinal application such as would healing effect, reduces blood sugar in diabetes, sooths burns, eases inpestiual problem, reduces arthritic swelling, ulcer curatine object, stimulates immunes response against cancer etc. Anthraquinotes derivatives in Aloe vera gel play an important role in the treatment of tumors, diabetes, ulcer and cancer [1,2,3] . Keeping this fact in view, the resent study was undertaken to isolate the phenolic anthroquinones from the methnolic extract from Aloe vera leaf gel. Materials and Methods The leaf, gel, root were collected from department of Horticulture, faculty of Agriculture, Annamalai University, Tamil Nadu and Commercial gel and commercial soap were collected Siddha Medical Stores. The five samples were collected. The leaf, gel, root, commercial gel and commercial soap samples were crushed and cold macerated in acetone for three days. The acetone was evaporated from the extract was filtered to clarity. The fixed extract thus obtained was tested for its FT-IR spectral studies. The three different treated samples of leaf, gel, root, commercial gel and soap were collected from department of Horiculture, faculty of Agriculture, Annamalai University and Retial Pharamaceutical stores. The sample are dissolved from acetone [4]. The spectra of all samples of leaf and stem are recorded under indentical conditions in the 4000 – 400 cm -1 region using fourier transform infrared spectrometer (spectrum RX-I, FT-IR system, perkineliner model) available in the CISL Lab or Department of Physics, Annamalai University. In the case of UV spectral measurements, the acetones extracts of five different sample of Aloe vera, leaf, gel, root, commercial gel and commercial soap were subjected to UV spectral analysis. It has been carried out using SH1MAD2U UV – 1650 spectrometer with a wavelength ranges at 200-600 nm. The UV spectrometer available in the Department of Chemistry, Annamalai University, Annamalai Nagar, Tamil Ndu has been used. Results and Discussion The Fourier Transform Infrared Spectra of all the leaf, Gel, Root, commercial gel and soap are shown in fig. (2.1 – 2.5).