Downloaded from www.microbiologyresearch.org by IP: 54.70.40.11 On: Tue, 11 Dec 2018 21:06:11 Devosia humi sp. nov., isolated from soil of a Korean pine (Pinus koraiensis) garden Juan Du, 1 3 MooChang Kook, 2 3 Shahina Akter, 1 3 Hina Singh, 1 KyungHwa Won, 1 Hien T. T. Ngo 1 and Tae-Hoo Yi 1 Correspondence Tae-Hoo Yi drhoo@khu.ac.kr 1 Department of Oriental Medicinal Material & Processing, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea 2 Department of Marine Biotechnology, Anyang University, Incheon 417-833, Republic of Korea A bacterial isolate, designated strain THG-MM1 T , was isolated from soil in Yongin region, Republic of Korea. Strain THG-MM1 T was Gram-stain-negative, aerobic, non-motile and rod- shaped. Based on 16S rRNA gene sequence comparisons, strain THG-MM1 T was most closely related to Devosia insulae DS-56 T (96.5 % 16S rRNA gene sequence similarity), followed by Devosia yakushimensis Yak96B T (95.7 %) and Devosia albogilva IPL15 T (95.2 %). The G+C content of the genomic DNA was 63.7 mol%. The major cellular fatty acids were C 16 : 0 , summed feature 8 (C 18 : 1 v7c and/or C 18 : 1 v6c) and C 18 : 0 . The predominant isoprenoid quinone was ubiquinone-10 (Q-10). The major polar lipids were phosphatidylglycerol and an unidentified lipid. The combined phenotypic, chemotaxonomic and phylogenetic data showed that strain THG-MM1 T represents a novel species of the genus Devosia, for which the name Devosia humi sp. nov. is proposed, with strain THG-MM1 T (5KACC 18281 T 5CCTCC AB 2015121 T ) as the type strain. The genus Devosia belongs to the class Alphaproteobacteria and family Hyphomicrobiaceae, and was created by the reclassification of ‘Pseudomonas riboflavina’ (Foster, 1944) as Devosia riboflavina (Nakagawa et al., 1996). The genus accommodates Gram-stain-negative, rod-shaped, aerobic, oxidase-positive bacteria containing ubiquinone-10 (Q-10) or Q-11 as the predominant respiratory quinone. The DNA G+C contents of members of the genus Devosia range from 59.5 to 66.2 mol% (Yoon et al., 2007; Jia et al., 2014). At the time of writing, the genus Devosia comprises 16 species with validly published names (http://www. bacterio.net/devosia.html). Various isolation sources have been reported of the genus Devosia including soil (Yoon et al., 2007), root nodule (Bautista et al., 2010), a hexachlorocyclohexane dump site (Verma et al., 2009), deep-sea sediment (Jia et al., 2014) and beach sediment (Lee, 2007). The genus Vasilyevaea belongs to the class Alphaproteobac- teria and was created by the reclassification of Prosthecomi- crobium enhydrum (Staley, 1968) as Vasilyevaea enhydra (Yee et al., 2010) and Prosthecomicrobium mishustinii (Vasil’eva et al., 1991) as Vasilyevaea mishustinii (Yee et al., 2010). The genus Vasilyevaea comprises two species with validly published names, which were isolated from water and soil, respectively (Yee et al., 2010). Mem- bers of the genus Vasilyevaea are Gram-reaction-negative, rod-shaped, aerobic bacteria and can utilize D-glucose and maltose as carbon sources. Cells of the genus Vasilye- vaea are irregular to thick rod-shaped and colony colours vary from white, yellow and red to yellow–orange. Mem- bers of the genus Vasilyevaea reproduce by budding and produce several cellular appendages or prosthecae that extend from each cell in all directions. The DNA G+C content of the genus Vasilyevaea ranges from 63 to 66 mol% (Yee et al., 2010). The genus Devosia is distinguished from the genus Vasilye- vaea by the cell shape and colony colour. The genus Devo- sia also differs from the genus Vasilyevaea by not reproducing by budding and prosthecae. In this study, we report on the characterization of a novel strain, THG-MM1 T , by using a polyphasic approach. On the basis of the results obtained, we propose that strain THG-MM1 T should be placed in the genus Devosia as the type strain of novel species. The soil sample was collected from a Korean pine (Pinus koraiensis) garden (378 149 270 N 1278 109 390 E) 3These authors equally contributed to this work. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain THG-MM1 T is KM598259. Three supplementary figures are available with the online Supplementary Material. International Journal of Systematic and Evolutionary Microbiology (2016), 66, 341–346 DOI 10.1099/ijsem.0.000724 000724 G 2015 IUMS Printed in Great Britain 341