© 2023 PP House Screening for In Vitro Antibacterial Activity of Leaves Extracts of Certain Selected Plants against Rhodococcus equi Lalit Kumar 1 , Laxmi Narayan Sankhala 1 , Lakshmi Kant 1 , Dinesh Kumar Badsiwal 2 , Sanjay Kumar 3 and Ramesh Kumar Dedar 4 Print ISSN 0976-3988 Online ISSN 0976-4038 Artcle AR3257 DOI: HTTPS://DOI.ORG/10.23910/1.2023.3257 Research Article International Journal of Bio-resource and Stress Management 1 Dept. of Veterinary Pharmacology and Toxicology, 2 Dept. of Epidemiology and Preventive Veterinary Medicine, College of Veterinary and Animal Science, RAJUVAS, Bikaner, Rajasthan (334 001), India 3 ICAR-NRCE, Hisar, Haryana (125 001), India 4 ICAR-NRCE, Equine Production Campus, Bikaner, Rajasthan (334 001), India RECEIVED on 12 th September 2022 RECEIVED in revised form on 14 th December 2022 ACCEPTED in fnal form on 01 st January 2023 PUBLISHED on 21 st January 2023 IJBSM January 2023, 14(1):101-109 htps://pphouse.org/ijbsm.php Citaton (VANCOUVER): Kumar et al., Screening for In Vitro Antibacterial Activity of Leaves Extracts of Certain Selected Plants against Rhodococcus equi . International Journal of Bio-resource and Stress Management, 2023; 14(1), 101-109. HTTPS://DOI. ORG/10.23910/1.2023.3257. Copyright: © 2023 Kumar et al. Tis is an open access article that permits unrestricted use, distribution and reproduction in any medium after the author(s) and source are credited. Data Availability Statement: Legal restrictions are imposed on the public sharing of raw data. However, authors have full right to transfer or share the data in raw form upon request subject to either meeting the conditions of the original consents and the original research study. Further, access of data needs to meet whether the user complies with the ethical and legal obligations as data controllers to allow for secondary use of the data outside of the original study. Confict of interests: Te authors have declared that no confict of interest exists. I n the present study, the research work was carried out during June 2018 to March 2019 at ICAR-NRCE-EPC (Indian Council of Agricultural Research, National Research Centre on Equines, Equine Production Campus), Jorbeer, Bikaner (Rajasthan), India. The study was conducted to investigate in vitro antibacterial activity of ethanolic, chloroformic and Sequentially Extracted Water Extract (SEWE) leaves extracts of Aegle marmelos, Morus alba, Nerium indicum, Cascabela thevetia (L.) Lippold, Plumeria alba L. and Azadirachta indica against Vap A and Vap C positive Rhodococcus equi. Fresh leaves of these plants were collected manually from campus of ICAR-NRCE-EPC, Jorbeer, Bikaner (Rajasthan). In initial screening ethanolic leaves extract of these plants except Azadirachta indica, were found non-active against Rhodococcus equi. Chloroformic leaves extracts of Azadirachta indica did not showed in vitro antibacterial activity against Rhodococcus equi. While ethanolic and Sequentially Extracted Water Extract (SEWE) leaves extracts of Azadirachta indica showed good in vitro antibacterial activity against Rhodococcus equi. Further, solvent based fractionation, Ethanol Soluble Fraction (ESF), Methanol Soluble Fraction (MSF) and Water Soluble Fraction (WSF) of polar compounds of SEWE did not showed in vitro antibacterial activity against Rhodococcus equi. On comparison with currently used antibiotics (azithromycin and rifampicin), required concentration of the leaves extract of Azadirachta indica was too high for their possibilities of in vivo use, so abundant availability of Azadirachta indica leaves and their activity against Rhodococcus equi suggests their potential for use as disinfectant against Rhodococcus equi. ABSTRACT Antibacterial, Azadirachta indica, in vitro, leaves, Rhodococcus equi KEYWORDS: Open Access allensankhala@gmail.com Corresponding 0000-0002-5340-4805 Stress Management 101