IJRPC 2020, 10(3), 305-313 Sridevi et al. ISSN: 22312781 305 INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com DOI: https://dx.doi.org/ 10.33289/IJRPC.10.3.2020.10(73) COMPARISON AND EVALUATION OF ANTIBIOTIC RESIDUES IN VARIOUS CHICKEN MEAT SAMPLES BY RP-HPLC METHOD M. Naga Hanusha, Pingali. Sridevi*, J. Kavitha, K. Vinutha, A. Mamatha and M. Bhagavan Raju Department of Pharmaceutical Analysis, Sri Venkateshwara College of Pharmacy, Madhapur, Hyderabad-500 086, Telangana, India. 1. INTRODUCTION Poultry industry is one of the biggest industries in India and globally in terms of production it is ranked 17th position. Accidentally, it was found that by- products of antibiotic production (dried streptomyces aureofaciens broth) which contain a high level of vitamin B12, when feed to poultry animals resulted in higher growth. Eventually, it was discovered that the trace amount of antibiotics remaining in these by- products accounted for high growth. Since then the antibiotics have been used on poultry in large quantities to enhance production in poultry. However, the use of antibiotics in food animals poses a major risk for humans due to antibiotic resistance. Antibiotic use is related to emergence of resistant bacteria in the animal which later transmits to human through food, environment and direct contact with the affected meat. Residues of antimicrobials compounds are also found in foods of animal ‘s origin as a Research Article ABSTRACT Antibiotics can be used in chicken meat as growth promoters and also to increase poultry production, but the antibiotics usage in chicken meat results into a major risk for humans dueto antibiotic resistance. In the present study ten chicken meat samples were collected from different regions of Hyderabad. Liquid-liquid extraction process was used for the extraction of Sulphamethoxazole, Acetonitrile, Acetone and Dichloromethane were used asorganic solvents. Microbiological method and was done for the determination of Sulphonamides in chicken meat samples. Thin layer chromatography was done to determine sulphamethaoxazole and other sulphonamides using Dichloromethane- methanol-water in the ratio of 69:35:6 as mobile phase. Retention factor (Rf) was calculated after chromatograms detected by spraying the plate with Fluorescamineand observed under Ultraviolet chamber. Quantification was done using RP-HPLCusing KROMOSIL 100 C18Column, 5μ, Mobile phase: di sodium hydrogen phosphate:methanol (75:25), Flow rate: 1 ml/min, Pump mode: isocratic, Run time: 10min, Column temperature: 25°CInjection volume: 20μl, Detection: UV at 266nm.Out of ten areas selected in Hyderabad region it was found that sulphonamide(sulphamethoxazole) concentration was more in Yakutpura and Mehdipatnam areas which are above MRLs (0.1μg/ml) (Maximum Residue Limit (MRL)recommended limit according to the European Union (EC). All the results showed that the developed method was accurate, simple and can used for the laboratory purpose for theanalysis of antibiotic residues in chicken meat. Keywords: Antibiotics, Chicken meat, poultry and sulphamethoxazole.