www.iajpr.com Page2808 Indo American Journal of Pharmaceutical Research, 2013 ISSN NO: 2231-6876 Journal home page: http://www.iajpr.com/index.php/en/ INDO AMERICAN JOURNAL OF PHARMACEUTICAL RESEARCH Evaluation of Invitro Antioxidant, Anti Inflammatory and Anti diabetic Potential of Curcumin Trilochan Satapathy 1 *, Prasanna Kumar Panda 2 1 Columbia Institute of Pharmacy, Tekari, Raipur, C.G, 493 111 2 University Department of Pharmaceutical Sciences, U. U., BBSR- 751 004 Corresponding author Dr.Trilochan Satapathy Associate professor and HOD, Dept of Pharmacology, Columbia Institute of Pharmacy, Raipur, C.G, Pin-493 111, Mob-7898369287, E-mail: trilochansatapathy@yahoo.co.in Copy right © 2013 This is an Open Access article distributed under the terms of the Indo American journal of Pharmaceutical Research, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. ARTICLE INFO ABSTRACT Article history Received 26/03/2013 Available online 30/03/2013 Keywords Curcumin; DPPH, Free Radical; Hyaluronidase; reducing power Curcumin, a yellow pigment from Curcuma longa, is a major component of turmeric and is commonly used as a spice and food-coloring agent. It is also used as a cosmetic and in some medicinal preparations. The desirable preventive or putative therapeutic properties of curcumin have also been considered to be associated with its antioxidant and anti-inflammatory properties. In the present study, we determined the antioxidant , anti inflammatory and anti diabetic (amylase inhibition) activities of curcumin by employing various in-vitro antioxidant assays such as Ferric reducing antioxidant power, Free radical scavenging activity, Hydroxyl radical scavenging activity, Nitric oxide radical scavenging activity and Amylase inhibition assay. The curcumin exhibited its radical scavenging effect in concentration dependent manner and the effect was compared with a known antioxidant agent (BHA/Ascorbic acid). The curcumin was also evaluated for its anti inflammatory activity by using hyaluronidase inhibition assay. The anti- inflammatory effect of curcumin is most likely mediated through its ability to inhibit cyclooxygenase-2 (COX-2), lipoxygenase (LOX), and inducible nitric oxide synthase (iNOS). COX-2, LOX, and iNOS are important enzymes that mediate inflammatory processes. Curcumin exhibited concentration dependent anti inflammatory activity. The anti-diabetic potential of curcumin was also evaluated by assessing amylase inhibition assay. The results for amylase inhibition activity reveal that the curcumin inhibits alpha-amylase in a concentration dependent manner. This study indicates significant amylase inhibition, free radical scavenging and hyaluronidase inhibition potential of curcumin which can be exploited for the treatment of various free radical mediated disease like inflammation and diabetes mellitus. The present study reveals that curcumin is a good antioxidant, anti inflammatory and anti diabetic agent hence, can be used in the pharmacological and food industry and as neutraceutical products. Please cite this article in press as Trilochan Satapathy et.al. Evaluation of Invitro Antioxidant, Anti Inflammatory and Anti diabetic Potential of Curcumin. Indo American Journal of Pharm Research.2013:3(3).