* Corresponding author: Michael Okey Enemali; Email: Department of Applied Biochemistry, Faculty of Biosciences, Nnamdi Azikiwe University, Awka – Nigeria. Copyright © 2021 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0. Effect of leaf extracts of Carica papaya (Paw paw) and Pakia biglobosa (African locust bean) on some biochemical parameters of albino rats Michael Okey Enemali 1, * , Kingsley Ikechukwu Ubaoji 1 , Chinenye Enoch Oguazu 1 , Gambo Sunday Haruna 2 and Kingsley Kelechi Asogwa 1 1 Department of Applied Biochemistry, Faculty of Biosciences, Nnamdi Azikiwe University, Awka – Nigeria. 2 Department of Biochemistry and Molecular Biology, Faculty of Natural and Applied Sciences, Nasarawa State University, Keffi – Ngeria. International Journal of Science and Technology Research Archive, 2021, 01(02), 014–021 Publication history: Received on 17 July 2021; revised on 08 September 2021; accepted on 10 September 2021 Article DOI: https://doi.org/10.53771/ijstra.2021.1.2.0033 Abstract In spite of the advances made in orthodox medicine, there has been an increasing interest in herbal medicine. The leaves of Carica papaya and Pakia biglobosa have been reported to contain lots of beneficial medicinal compounds, hence their use in the traditional prevention, management and treatment of ailments/diseases. In this study, the effect of varied concentrations of the ethanol leaf extract of the plants on some biochemical parameters of albino rats was assessed. The phytochemical compositions of the leaves were determined using established standard laboratory methods. Fifty four male Albino rats weighing between 150g-200g were randomly distributed into nine groups of six animals each. A daily single dose of 500mg/kg, 1000mg/kg, 1500mg/kg and 2000mg/kg body weight of either of the extracts was respectively, administered to the eight test groups for fourteen days. The control group was given only feed and water. Biochemical parameters such as the serum activities of Aspartate Aminotransferase, Alanine Aminotranferase and Alkaline phosphatase as well as the serum concentrations of Bilirubin, Albumin, HCO3 - , Urea, Creatinine and Na + were assessed. Result of the analyses showed that the administration of the extracts did not significantly raise the serum activity of ALT. The decreases in serum concentrations of Urea and increase in the concentration of HCO3 - were directly proportional to the concentration of the extracts. It can be deduced from this study, that 500mg, 1000mg 1500mg and 2000mg/kg bw of either C. papaya or P. biglobosa did not elicit any marked hepatotoxicological or renotoxicological effect on the experimental animals Keywords: Carica papaya; Pakia biglobosa; Aminotransferases; Hepatotoxicological; Renotoxicological; Biochemical parameters 1. Introduction Toxicology is the study of poisons, or, more comprehensively, the identification and quantification of adverse outcomes associated with exposure to physical agents, chemical substances and other conditions. The fundamental goal of toxicology is to determine safe levels of exposure to potentially poisonous substances for humans and the environment [1]. Plants produce most of the world’s oxygen, and are important in the food chain, as many organisms eat plants or eat organisms which eat plants. Plants that possess therapeutic properties or exert beneficial pharmacological effects on the human body are generally designated as medicinal plants. However, most of these plants used as traditional medicines by locals are not subjected to laboratory analysis or do undergo any purification processes apart from the washing and boiling and even extraction process. Dosage forms, side effects and efficacy of most of these medicinal plant preparations are usually not clearly defined, despite the common and frequent use for therapy, based on the belief that