www.electrophoresis-journal.com Page 1 Electrophoresis Received: 22-Jul-2014; Revised: 24-Sep-2014; Accepted: 02-Nov-2014 This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.1002/elps.201400356. This article is protected by copyright. All rights reserved. Optimized On-Line Enantioselective Capillary Electrophoretic Method for Kinetic and Inhibition Studies of Drug Metabolism Mediated by Cytochrome P450 Enzymes Roman Řemínek 1,2 , Zdeněk Glatz 1 , Wolfgang Thormann 2 * 1 Department of Biochemistry, Faculty of Science and CEITEC – Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic 2 Clinical Pharmacology Laboratory, Institute for Infectious Diseases, University of Bern, Murtenstrasse 35, 3010 Bern, Switzerland E-mail: wolfgang.thormann@ifik.unibe.ch, Tel: +41-31-632-32-88, Fax: +41-31-632-4997 Keywords: Capillary electrophoresis; Cytochrome P450 3A4; Dexmedetomidine; Enantioselective drug metabolism; Ketamine; Ketoconazole List of abbreviations: CYP, cytochrome P450; CYP3A4, cytochrome P450 3A4 isoform; EMMA, electrophoretically mediated microanalysis; IC 50 , half maximal inhibitory concentration; K a ’, apparent constant of autoactivation; K i ’, apparent inhibition constant; K m ’, apparent Michaelis-Menten constant; LADME/Tox, liberation, absorption, distribution, metabolism, excretion, toxicity; n, Hill coefficient; NADPH, β-nicotinamide adenine dinucleotide phosphate reduced; SE, standard error; V max ’, apparent maximum reaction velocity