METASTATIC TUBULOPAPILLARY MAMMARY CARCINOMA IN A LEOPARD (PANTHERA PARDUS) I.C.  So stari c-Zuckermann * , B. Artukovi c * , O. Smolec y , A.Gudan Kurilj * , D. Huber * ,  Z. Grabarevi c * and M. Hoh steter * * Department of Veterinary Pathology and y Surgery, Orthopedics and Ophthalmology Clinic, Veterinary Faculty, University of Zagreb, Zagreb, Croatia Introduction: Mammary tumours are common and usually malig- nant in domestic cats and are also recognized in wild felids. This report presents a case of metastatic mammary carcinoma in a leopard. Materials and Methods: A multilobulated mammary mass was excised from a 16-year-old, female leopard from Zagreb Zoo and sub- mitted for evaluation. About 1 year later, the animal died and nec- ropsy examination was performed. Routine HE staining and IHC with Ki67 and E-cadherin were used on the initial tumour sample and also on tumour masses collected at necropsy. Results: Microscopical examination of the initial tumour revealed an infiltrative tubulopapillary mass that invaded the lymphatic vessels. Mitotic index was moderate (15 per 10 HPFs). The tumour was clas- sified as tubulopapillary carcinoma of the mammary gland. At nec- ropsy examination, the animal was emaciated. Numerous nodular metastases were evident in the lymph nodes, liver, uterus and spleen. Smaller microscopical metastases were found in the lung. The histo- logical appearance of the metastases was comparable with the pri- mary tumour. In the primary tumour, the Ki67 proliferation index was 28.7% and E-cadherin expression was 56.8%. In the metastases, Ki67 and E-cadherin expression averaged 17.1% and 41.9%, respectively. Conclusions: This case depicts an otherwise common feline tumour in a large felid. Expression of E-cadherin shows a small drop in the metastases suggesting additional anaplasia of the metastatic tumour cells. The slight drop in the Ki67 expression could be due to more widespread necrosis in the metastases. This case postulates that mam- mary tumours in wild felids have similar clinicopathological features to those in domestic cats. RENAL NEPHROBLASTOMA IN A GREEN ANACONDA (EUNECTES MURINUS) M. Tecilla * , W. Magnone y , C. Sandri y and P. Roccabianca * * DiMeVet, University of Milan, Via Celoria 10, Milan and y Parco Natura Viva, Garda Zoological Park srl, Localit  a Figara 40, Bussolengo, Italy Introduction: Nephroblastoma is a renal tumour reported in higher and lower vertebrates. In higher vertebrates, nephroblastoma is more often a paediatric tumour arising from metanephric rests. In contrast, in lower vertebrates, nephroblastoma derives from the opistonephros (mature kidney). Nephroblastomas are rare in snakes. The aim of this report is to document the second case of nephroblastoma in an anaconda. Materials and Methods: A 20-year-old, green, male anaconda was evaluated ultrasonographically in 2012 for severe coelomic distension caused by a bilateral renal mass. The animal was monitored yearly and due to the progressive increase of tumour size and development of anorexia, was humanely destroyed in 2017. Results: The left kidney was replaced by a 3 kg, multilobulated tanewhite neoplasm and was surrounded by a large haematoma. Histologically, a triphasic tumour composed of embryonic mesen- chyme, primitive glomeruli and nests of polygonal cells (blastema) was observed. A definitive diagnosis of nephroblastoma was made. Conclusions: Nephroblastomas have been reported anecdotally in adult snakes and described only in one anaconda. However, nephro- blastoma in snakes is a senile tumour since, contrary to mammals and birds, postembryonic nephrogenesis occurs in reptiles (including Corn snakes). The WT1 pathway is involved in renal development and increased WT1 expression has been associated with the onset of renal neoplasia, such as nephroblastoma, in several mammals and in renal cell carcinoma only in man. NUTRITIONAL MUSCULAR DYSTROPHY IN CHINCHILLA LANIGERA T.B. Lagoa * , E.F. Bondan * ,y and S.V. Santos * * Department of Veterinary Medicine, University Cruzeiro do Sul and y Department of Environmental and Experimental Pathology, University Paulista (UNIP), S~ ao Paulo, Brazil Introduction: Nutritional muscular dystrophy (NMD), also known as white muscle disease, is a subacute or acute disease of the cardiac and/or skeletal striated musculature of multifactorial origin, although frequently associated with deficiency of selenium and vitamin E in di- etary intake. Deficiencies of these nutrients can lead to oxidative cell damage as they are important as reactive oxygen species scavengers. Chinchillas include two species of crepuscular rodents that are native to the Andes mountain range in South America. The two living spe- cies of chinchilla are Chinchilla chinchilla and Chinchilla lanigera. C. lani- gera has a longer tail and ears and thinner neck than C. chinchilla. Domesticated chinchillas are thought to have come from the C. lani- gera species. Little is known about the minimum nutritional require- ments for these rodents and, sometimes, nutrient composition is not even discriminated in commercial food rations for the species. Materials and Methods: A 10-month-old, male specimen of C. la- nigera raised in captivity was submitted for necropsy examination af- ter presenting with signs of cardiorespiratory decompensation, muscular weakness and permanent decubitus. Tissue samples were collected for histopathological examination. Results: Microscopical analysis revealed segmental rhabdomyolysis (myonecrosis, inflammation and increased endomysial connective tis- sue) and dilated cardiomyopathy, severe pulmonary oedema and moderate renal tubular necrosis. Histopathological findings and the history of poor nutritional management were fundamental to estab- lish the diagnosis for NMD. Conclusions: To our knowledge this is the first description of the dis- ease in chinchillas and it aims to alert clinicians who deal with exotic animals about the susceptibility of these rodents to NMD. REPEATABILITY OF MYODEGENERATION SCORING IN BROILER WOODEN BREAST MYOPATHY H.-K. Sihvo * , N. Airas * , O. Simola y and D.K. Meyerholz z * Department of Veterinary Biosciences, University of Helsinki, y Orion Corporation Orion Pharma, Finland and z Department of Pathology, University of Iowa, Iowa City, IA, USA Introduction: Myodegeneration is the main histological finding in wooden breast myopathy (WB), which is macroscopically denoted by hardened muscle consistency and pale colour of the pectoralis ma- jor muscle in broiler chickens. A recent publication suggested a four- tier scoring of myodegeneration in the WB diagnosis. The aim of this study was to evaluate the interobserver repeatability of the scoring and the correlation of the re-scored values with the macroscopic WB classification. Materials and Methods: Histological sections of broiler chicken pectoralis major muscles (n 5 25) were retrieved from the original scoring study material published previously. The samples were chosen by stratified random fashion, representing macroscopically unaffected and WB cases and all histological scores (0e3). Two veterinary pa- thologists scored the samples based on the previously published scoring criteria. The pathologists were comprehensively masked to the macroscopic WB classification. Results: The interobserver repeatability of the scoring between the original scoring and pathologist A was good (Cohen’s k 5 0.67, P !0.0005) and moderate for pathologist B (k 5 0.53, P ! 0.0005). Spearman’s correlation between the histological scoring and the macroscopic WB classification was O0.8, P !0.0005 for all pathologists (original and re-scoring). Conclusions: The WB myodegeneration scoring criteria yields mod- erate to good repeatable results between pathologists and the degen- eration scores strongly correlate to the macroscopic appearance of the muscle. Unlike in other species, minimal myodegeneration typically occurs also in macroscopically unaffected broiler chickens and the evaluation of repeatability highlights the importance of a detailed scoring criteria description in publications. J. Comp. Path. 2018, Vol. 158, 93e149 ESVP, ESTP and ECVP Proceedings 2017 147