Journal of Progressive Research in Chemistry [JPRC] ISSN 2454-3136 Volume 4, Issue 3 available at www.scitecresearch.com/journals/index.php/jprc 194 SCITECH Volume 4, Issue 3 RESEARCH ORGANISATION May 18, 2017 Journal of Progressive Research in Chemistry www.scitecresearch.com Photochemical Investigation of the Roots of Jatropha Integerrima Jacq Surinder Kumar Sharma 1, 3 , Mohammed Ali 2 *, Harneet Singh 1 , Shahnaz Sultana2, 4, Showkat R. Mir 2 1 Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar – 125 001 (Haryana), India 2 Phytochemistry Research Laboratory, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110 062, India. 3 Present address: Herbal Drug Technology, Delhi Pharmaceutical Science and Research University , Puspvihar, Sector 3, New Delhi 110 017, India. 4 Present address: College of Pharmacy, Jazan University, Jazan, Saudi Arabia. Abstract Jatropha integerrima Jacq. (Euphorbiaceae) is grown in many tropical and subtropical countries and used to treat eczema, herpes, ringworm, rheumatism, pruritus, scabies, toothaches, tumors and warts. Its latex is toxic. A methanolic extract of the roots was chromatographed over silica gel column packed in petroleum ether. The column was eluted with petroleum ether, chloroform and methanol successively in order of increasing polarity to isolate n-pentadecanyl oleate (1), α-D-glucopyranosyl-(2→1’)-O-α-D-glucopyranoside (α-D-diglucoside, 2), n-hexanoyl-O- α-D-glucopyranosyl-(2a→1b)-O- α-D-glucopyranosyl-(2b→1c)-O- α-D-glucopyranosyl-(2c→1d)- O- α-D-glucopyranosyl-(2d→1e)-O- α-D-glucopyranoside (n-caproyl O-α-D-pentaglucoside, 3), n-hexanoyl-O- β-D-glucopyranosyl-(2a→1b)-O-β-D-glucopyranosyl-(2b→1c)-O-β-D-glucopyranosyl-(2c→1d)-O-β-D- glucopyranosyl-(2d→1e)-O-β-D-glucopyranosyl-(2e→1f)-O-β-D-glucopyranoside (n-caproyl O-β-D- hexaglucoside, 4), α-D-glucopyranosyl-(2→1a)-O- α-D-glucopyranosyl-(2a→1b)-O- α-D-glucopyranosyl- (2b→1c)-O- α-D-glucopyranosyl-(2c→1d)-O- α-D-glucopyranosyl-(2d→1e)-O- α-D-glucopyranoside (α-D- hexaglucoside 5) and α-D-glucopyranosyl-(2→1a)-O- α-D-glucopyranosyl-(2a→1b)-O- α-D-glucopyranosyl- (2b→1c)-O- α-D-glucopyranosyl-(2c→1d)-O- α-D-glucopyranosyl-(2d→1e)-O- α-D-glucopyranosyl-(2e→1f)-O- α-D-glucopyranoside (α-D-heptaglucoside, 6) .The structures of these compounds have been established on the basis of spectral data analysis and chemical means. Keywords: Jatropha Integerrima; Roots;Chemical Constituents; Isolation; Characterization. 1. Introduction Jatrophaintegerrima Jacq., syn. J. acuminate Desr., J. coccinea Link, J. diversifolia A.Rich., J.pandurifolia Andrews(Euphorbiaceae), commonly known as peregrina, spicy jatropha, firecracker jatroph and coral plant, is a native to the West Indies, Cuba, Dominican Republic and Haiti, but widely grown for ornament in many tropical and subtropical countries including southern India, Philippines. Thailand and South America. It is a slender-stemmed, multi-trunked tropical evergreen tree or large shrub, up to 5 m in height with an equal spread, and has unusual long glossy green, oblong, fiddle-shaped, or even-lobed leaves with beautiful clusters of scarlet flowers and seeded capsules [1-3]. It should be kept out of the reach of children. It is related to Jatropha cruces that are grown commercially to produce oil used in biodiesel fuel. The plant is used as an emetic, purgative, styptic and to treat eczema, herpes, ringworm, rheumatism, pruritus, scabies, toothaches, tumors and warts [4, 5]. Its latex is toxic, the leaves are purgative and if accidentally chewed, can cause squeamish and stomachalgia [6]. The latex contained cyclic peptides, integerrimides A, B and C [5, 7 - 9]. The leaf essential oil was composed of pentad canal, 1, 8-cineole, and β-ionone as the major components. The seed oil was comprised mainly of aliphatic hydrocarbons pentacosane, hexacosane, octacosane and heptacosane and fatty acids viz., palmitic, ricinoleic, steraric, oleic, linoleic, linolenicand arachidic acids [5]. However, the defatted seed oil was consisted of the monoterpenes 1, 8-cineole, p-cymene and α-pinene [10]. The trunks possessed sesquineolignan enantiomers, jatrointelignans A and B, neolignans jatrointelignans C and D and schisphenlignan [11]. The roots yielded