IOSR Journal of Applied Chemistry (IOSR-JAC) ISSN: 2278-5736. Volume 3, Issue 2 (Nov. – Dec. 2012), PP 21-29 www.iosrjournals.org www.iosrjournals.org 21 | Page Preparation, Characterisation and Anti-fungal activity of 1E-1- (1H-Benzimidazol-2-yl)-N-hydroxy ethanimine and 1E-1-(1H- Benzimidazol-2-yl)-N-hydroxy-1-phenyl methanimine with Co(II), Ni(II), Cu(II), Zn(II) and Cd(II). Madhu Bala 1 ; Khursheed Ahmad 1 ; S. R. Sharma 1 and L.K. Mishra 2 1 Department of Chemistry, NIT Patna 2 Department of Chemistry, Science College, Patna Abstract: The complexes of Co(II), Ni(II), Cu(II), Zn(II) and Cd(II) with 1E-1-(1H-Benzimidazol-2-yl)-N- hydroxy-ethanimine (Hbhe) and 1E-1-(1H-Benzimidazol-2-yl)-N-hydroxy-1-phenylmethanimine (Hbhpm) of compositions [M(HL) 2 Cl 2 ], (M=Co II , or Ni II and HL=Hbhe or Hbhpm), [M(HL)X 2 ], (M=CuII, ZnII or CdII, HL=Hbhe or Hbhpm and X=Cl or Br ) and ML 2 .nH 2 O, (M=Co II , Ni II , Cu II , Zn II or Cd II , HL=Hbhe or Hbhpm and n= 0 or 2 ) have been prepared and characterised by electrical conductance value, magnetic susceptibility and spectral studies. The anti- fungal activity of ligands and neutral bis-chelates ML 2 .nH 2 O was investigated by growth inhibition method. The complexes exhibited enhanced activity than free ligands. Key Words: Anti-fungal activity, Benzimidazolyloxime, Chacterisation, Metal complexes, Synthesis. I. Introduction In previous communication, we have reported the spectral and structural aspects of metal complexes of benzimidazole and substituted benzimidazoles in solid state 1-4 .The analytical uses, industrial characteristics and wide spectrum of pharmacological activity of benzimidazole derivatives created huge interest during last few decades to investigate microbial activity of new benzimidazole derivatives 5-10 . Extensive biochemical studies have confirmed that various benzimidazole derivatives are effective against strains of microorganisms 5-6 . Certain derivatives exhibit the biosynthesis of Ergosterol in the cell membrane of Fungi 7 .In addition to potential use of benzimidazole derivatives for treatment of bacterial, viral, fungal, HBV and parasitic diseases as effective and selective medicines 7-19 . A number of its derivatives produce temperature resistant electrical conducting polymer 20 .In present communication we report synthesis characterisation and anti-fungal activity of Hbhe and Hbhpm and their complexes with Co II , Ni II , Cu II , Zn II and Cd II . II. Method and Materials Metal halide and acetate used were E. Merck Extra pure or BDH Anal R grade chemicals. Ortho- phenylenediamine, lactic acid, Mendalic acid, DMF, organic solvents were either obtained from E.Merck or Nice and Sd. Fine chemicals. The ligands, Hbhe and Hbhpm were prepared by condensing 2- Acetylbenzimidazole [1-(1H-benzimidazol-2-yl)ethanone] and 2-Benzoylbenzimidazole [1-(1H-benzimidazol- 2-yl)phenone] with hydroxylamine hydrochloride in aqueous ethanol in basic medium using K 2 CO 3 . 2- Acetylbenzimidazole and 2-Benzoylbenzimidazole were synthesized by reported method 21, 22 . The ligands separated as white crystalline precipitate by neutralising the reactants with dilute hydrochloric acid. The ligands were analysed. For Hbhe, Nitrogen found 23.73% required for C 9 H 9 N 3 O, 24% for Hbhpm, Nitrogen found 17.68%, required for C 14 H 11 N 3 O, 17.72% m.pt of Hbhe-243 0 c and that of Hbhpm-195 0 c. Metal and halogen of complexes were determined by standard procedure 23 .Results of C, H and N analysis were obtained from CDRI Lucknow by Gouy method at room temperature (304-305K). Infrared spectra of compounds as KBr disc in the range of 4000-400 cm -1 on SHIMADZU- FTIR spectrophotometer at IIT Patna. The U-V spectra in ethanol or DMF solution of ligands and complexes were recorded in the range 200- 550 nm or 200-850 nm at IIT Patna. The NMR spectra were recorded on Brucker DRX FT-NMR spectrometer (300MHz) in DMSO or CDCl 3 . Mass spectra of ligands were recorded at IIT Patna. The molar electrical conductance values were measured at room temperature (30-31 0 c) by Digital conductivity meter of model 611E having cell constant- 1, using 10 -3 molar solution of complexes in DMF solution. 2.1 Preparation of complexes ML 2 .nH 2 O (M= Co II ,Ni II ,Cu II ,Zn II or Cd II , HL=Hbhe or Hbhpm and n= 0 or 2) About 5 millimole of metal acetate or chloride was dissolved in 25-30 ml hot aqueous ethanol and added with constant stirring to hot ethanol solution of 10 millimole of appropriate ligand (30 ml). The mixed solution was heated on steam bath by adding 20-25 ml (5%) aqueous solution of potassium acetate. The neutral bis-chelates