Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Jurnal Gizi Indonesia Vol. 9, No. 2, June 2021 (136-142) Submitted: 19 February 2020, Accepted: 3 April 2021 Online https://ejournal.undip.ac.id/index.php/jgi 1 Jurusan Gizi, Poltekkes Kemenkes Kupang 2 Jurusan Gizi, Poltekkes Kemenkes Aceh *Correspondence : e-mail: juntra8686@gmail.com, Telp/HP. 081338666698 Copyright © 2021; Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition), Volume 9 (2), 2021 e-ISSN: 2338-3119, p-ISSN: 1858-4942 136 Effects of mixture powder of black rice (Oryza sativa L indica), red beans (Phaseolus vulgaris L), and moringa leaves (Moringa oleifera L) on blood glucose concentration in hyperglycemic Rats Lalu Juntra Utama 1* , Suryana Suryana 2 , Anita Christina Sembiring 1 ABSTRACT Background: Diabetes mellitus, increased blood glucose or hyperglycemia, is associated with increased oxidative stress and cardiovascular diseases. This condition will further cause carbohydrate and fat metabolism change, resulting in the decreased antioxidant defense system. Black rice, red beans, and moringa leaves contain oleic acid, butyric amino acid, antioxidants, phytic acid, and arginine, which can improve insulin sensitivity, and blood glucose homeostasis. Objective: This study aimed to analyze the effect of betamelor (black rice, red beans, and moringa leaves) on blood glucose in rats Materials and Methods: The design of this research was experimental research with pre-and post-control group design. A total of 20 Sprague Dawley female rats were divided into four groups, namely standard feed (PS), 80% feed of betamelor (PB8), 50% feed of betamelor (PB5), and 20% feed of betamelor (PB2). Betamelor intervention was given as much as 5% of weight for 28 days. Fasting Blood Glucose (FBG) levels were measured using the GOD-PAP method. Blood glucose data were analyzed by Analysis of Variance (ANOVA) at a 95% confidence level and using Duncan's test. Results: There were differences in FBG between groups after the intervention of betamelor. The results showed that after 28 days of intervention, betamelor decreased the serum glucose concentration from 122.69 mg / dL to 97.70 mg / dL (20.37%) in the PB8 group and from 123.91 mg / dL to 113.28 mg / dL (8.58%) in the PB5 group, but the standard diet (PS) increased by 5.73%. This result can be applied to reduce blood glucose levels in obese and patients with metabolic syndrome. Conclusions: There was a significant effect of giving a mixture of black rice, red beans, and Moringa leaves on fasting blood sugar in rats. Keywords: Black rice; Red beans; Moringa leaves; Fasting blood glucose BACKGROUND The changes in lifestyle and diet of carbohydrate-based foods into high-fat content foods leads to the rise of degenerative diseases such as coronary heart disease, hypertension, and diabetes mellitus. An increase in blood glucose or hyperglycemia is a sign of a metabolic disorder known as diabetes mellitus and is associated with the rise of oxidative stress and complications in the vascular system. It will further cause the alteration in carbohydrate and fat metabolism resulting in an impairment of the oxidation defense system. In which this condition stimulates the increased formation of Reactive Oxygen Species (ROS) induce β-pancreatic cell dysfunction.1 One of the antioxidant defense systems is Superoxide Dismutase (SOD), the enzyme involved in the earliest and most potent detoxification in cells.2 The antioxidant enzyme itself is an essential enzyme which is able to eliminate radicals, then may protect cells against toxic of aerobic metabolism byproducts.3 The use of oral medicine of diabetes mellitus accompanied by the use of natural ingredients already become common practice yet, and around 1050 anti-Diabetes Mellitus plants have been studied.4 Some plants commonly used as a source of functional foods and already developed to help in controlling blood sugar are black rice, red beans, and moringa leaves. Ingredients contain oleic acid, amino butyric acid, antioxidants, pitic acid and arginine which are proven to reduce the rate of oxidative stress, thereby increasing antioxidants in the body. Following Walter and Marchesan (2011), phenolic compounds are concentrated higher in the black rice pericarp.5 Bioactive compounds work as antioxidants in rice, including phenolic, flavonoid,