~ 282 ~  Journal of Pharmacognosy and Phytochemistry Journal of Pharmacognosy and Phytochemistry 2015; 4(2): 282-291 E-ISSN: 2278-4136 P-ISSN: 2349-8234 JPP 2015; 4(2): 282-291 Received: 21-05-2015 Accepted: 25-06-2015 Parul Namdev Food Processing Technology Laboratory, USBT, Guru Gobind Singh Indraprastha University, Sector-16 C, Dwarka, New Delhi- 110078, India. Rajinder K Gupta Food Processing Technology Laboratory, USBT, Guru Gobind Singh Indraprastha University, Sector-16 C, Dwarka, New Delhi- 110078, India. Email: rkg67ap@yahoo.com. Contact No : +91-11-25302321 Correspondence: Rajinder K Gupta. Food Processing Technology Laboratory, USBT, Guru Gobind Singh Indraprastha University, Sector-16 C, Dwarka, New Delhi- 110078, India. Email: rkg67ap@yahoo.com, Contact No : +91-11-25302321 Herbal green tea formulation using Withania somnifera stems, Terminalia arjuna bark, Cinnamon bark and Tinospora cordifolia stems and nutritional & phytochemical analysis Parul Namdev, Rajinder K Gupta Abstract Tea in general and Green tea in particular, are gaining increasing consumer attention due to a growing awareness of health benefits derived from their use, but research in product development of flavored Green tea is limited. The objectives of the study were to conduct Nutritional analysis, phytochemical analysis, antioxidant and antibacterial activity of Withania somnifera stems, Cinnamon bark, Tinospora cordifolia stems, Terminalia arjuna bark, Green tea and formulation mixture of these herbs in order to assess their potential for new herbal tea development. These herbs are a rich source of natural antioxidants and polyphenols. Total phenolics and flavonoids contents were obtained for methanolic extracts for each herb sample. Antioxidant activities of the extracts were estimated using DPPH radical scavenging activity and FRAP assay. Formulation mixture is an excellent sources of calcium, magnesium and phosphorus. The methanolic extracts showed good antibacterial activity in Bacillus subtilis, Bacillus cereus and E.coli. GC/MS screening showed the presence of several useful compounds indicating that the samples hold the potential to be stated under “Nutraceuticals”. HPLC method was developed for the estimation of quercetin, kaempferol, rutin & epicatechin in the formulation mixture extract. Formulation of green tea with these herbs was done, which imparts colour, aroma, flavor, astringency and overall acceptability to Green tea and impart many health benefits. Keywords: Withania somnifera stems, Cinnamon bark, Tinospora cordifolia stems, Terminalia arjuna bark, Nutritional, Antioxidant, Phytochemical, Antibacterial, GC/MS 1. Introduction The Tea is the most consumed beverage in the world, but its origination is attributed to china [1] and becomes as an important food product in the world. After china, the 2 nd largest producer of tea is India [2] . These days consumers are cautious of their health so they are demanding for more natural and health benefitting food so tea seems to be a good vehicle in this respect because of its good taste and aroma. Hence tea belongs to a quickly growing market of wellness beverages [3] . Traditionally, tea is classified as green, Oolong, black and herbal tea [4] and can be differentiated on the basis of their processing stages at the time of manufacturing. Camellia sinensis is the plant from which green tea and all kinds of tea are made. Fermentation is not done during green tea Processing. Green tea imparts several health promoting components because of its high range of phenolic compounds. Green tea is covered to deliver nearly 4000 bioactive compounds under which one third is polyphenols [5] .Tannins and flavonoids are the important polyphenols present in the Green tea. Catechins, one of the important flavonoid present in the green tea which is also known as vitamin P [6] . Green tea imparts several health benefits like weight loss, diabetes, heart diseases, alzheimer’s and parkinson’s, blood pressure, depression, anti-viral, skin care. There are many flavored green teas. Popular flavored green teas are lemon green tea, ginger & mint green tea, lemon honey green tea, jasmine green tea, etc. It is thus imperative to research the potential of native plant materials in the expansion of new flavored green tea. On the basis of some unpublished reports, however, green tea imparts poor in sensory appeal due to the lack of distinct Flavor properties. Therefore, it may be needed to blend green tea with other herbs as a means of improving its sensory appeal and for good health and wellness. The herbs discussed in the research work are Withania somnifera stems, cinnamon bark, Tinospora cordifolia stems and Terminalia arjuna bark. Withania somnifera (L) Dunal is fit to known as Indian medicinal plant which is usually used in the healing of many clinical conditions in India, also known as “Ashwagandha” contains a broad variety of significant substances in our bodies. It contains a large amount of steroidal