LJBS, 2021 https://www.raftpubs.com/ Volume 5 | Issue 1 | pg. 46 Research Article E-ISSN: 2707-6261 RAFT Publications Phytochemical Investigation and Spectrophotometric Studies of Piperine and Thymoquinone Compounds in Methanolic Extracts of Nigella sativa and Piper nigram spices Hamad. M. Adress. Hasan 1 and Eman. I. Hassan. Eljadily 2* 1 Chemistry Department, Faculty of Science, Omar Al-Mukhtar 2 Chemistry Department, Faculty of Arts and Science, Omar Al-Mukhtar *Correspondence: Eman. I. Hassan. Eljadily, Chemistry Department, Faculty of Arts and Science, Omar Al-Mukhtar, Email: emaneljadily1971614@Gmail.com Received: 15 March 2021 Accepted: 13 April 2021 Published: 19 April 2021 DOI: https://doi.org/10.36811/ljbs.2021.110065 Citation: Faraj. A. Abdunabi. 2021. Phytochemical Investigation and Spectrophotometric Studies of Piperine and Thymoquinone Compounds in Methanolic Extracts of Nigella sativa and Piper nigram spices. LJBS. 5: 46-54. Abstract This study was carried out on some spice samples including Nigella sativa and Piper nigram. Phytochemical screening was applied to the methanolic extraction of both the studied plants. The results showed that methanolic extract of Nigella sativa and Piper nigram revealed the presence of various chemical constituents, such as Alkaloide, Carbohydrates, Glycosides, Tannins, Flavones, Saponins. Piperine is characterizing compound present in fruits of piper nigram used as a bioavailability enhancer. Some spectrophotometric methods as (U.V) and I.R were applied to identify the major chemical compounds of the studied extracts. The results recorded that the Piperine and Thymoquinone compounds were found in the studied plant extracts. Keywords: Piper Nigram, Nigella sativa, Piperine Thymoquinone, Spectrophotometric Introduction The Plants are significant wellsprings of medication utilizing in all societies from antiquated occasions. The different native plants are being utilized in the analysis, counteraction, and disposal of physical, mental, or social irregularity [1]. Restorative plants are the foundation of customary medication and are vital to the wellbeing of people and networks [2]. The restorative estimation of these plants lies in some synthetic substances that produce a distinct physiological activity on the human body [3]. The medications are gotten from the entire plant or from various organs, similar to leaves, stem, bark, root, blossom, seed, and so on. Some medications are set up from excretory plant items, for example, gum, Lybian Journal of Basic Sciences