This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-Non Commercial- ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. © 2023 Journal of Advanced Pharmacy Education & Research | Published by SPER Publication 118 Regular donor characteristics, inter-donation interval and the presence of subclinical anemia – A 3-year observational single- center study Teguh Triyono 1* , Komang Agus Trisna Amijaya 2 1 Departement of Clinical Pathology and Laboratory Medicine, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia. 2 Clinical Pathology Specialist Program, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia. Correspondence: Teguh Triyono, Departement of Clinical Pathology and Laboratory Medicine, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia. teguhpk@ugm.ac.id ABSTRACT Regular blood donation is one of the potential causes of iron loss in donors. Understanding the primary causes of subclinical anemia will aid in promoting the well-being of blood donors and improve the steadily diminishing donor pool. This study aims to analyze the effect of regular donations and investigate the incidences of subclinical anemia and their essential risk factors. A retrospective analytic observational study was used, from January 2016-December 2019. Demographic information was collected. Males with a hemoglobin level between 13 and 13.9 g/dL and females with a hemoglobin level between 12 and 12.9 g/dL are considered to have subclinical anemia. The data were analyzed with comparative, bivariate, and regression tests using the SPSSv25 with a significance of p-value<0.05. The study included a total of 4,629 regular voluntary blood donors. The mean age was 31.2 ± 9.87 years (17–65), and the weight was 67.96 ± 12.40 kg. The subclinical anemia of male and female subjects was 25.9% vs. 35.4%. Donors with subclinical anemia were female (OR=1.567, p<.001), weight ≤ 63.5 kg (OR=1.527, p<0.001), inter-donation intervals 8-21 weeks (OR=1.447, p<0.001), previous donation hemoglobin levels ≤ 14.75 g/dL (OR=3.051, p<0.001), and several donations ≥ 6 times (OR=1.533, p<0.001). Risk of subclinical anemia was female, weight ≤ 63.5 kg, inter-donation interval 8-21 weeks, previous donation hemoglobin level ≤ 14.75 g/dL in males or ≤ 14 g/dL in females, and several donations ≥ 6 times. Keywords: Regular donor, Demographic characteristics, Inter-donation interval, Hemoglobin levels, Subclinical anemia Introduction Blood transfusion is a central component of the medical and surgical care of patients [1-4] in all nations' healthcare systems. Its practice relies on the secure transfusion of blood from healthy donors to patients [5, 6]. Blood transfusions are only effective if the transfused blood is of high quality and the donor is secure. Regular blood donation is associated with donor iron deficiency [7]. Regular blood donation can contribute significantly to iron deficiency in blood donors. The movement of iron from body stores is caused by bleeding [8]. A person can either remain stable with low iron stores or become anemic during regular blood donations. Iron deficiency is commonly observed among long- term, consistent blood donors [9]. There has been a regulation of blood donation frequency to prevent anemia in the majority of donors, but quantitative data remains scarce [10, 11]. The screening of a donor's hemoglobin level is a standard component of the donor selection procedure to ensure that the red blood cells collected are of sufficient quality and that the donor is healthy [12]. Strategies to prevent hemoglobin deficiency and iron deficiency anemia, including donor reduction, have been implemented in many individual institutions [13]. In general, the minimum Access this article online Website: www.japer.in E-ISSN: 2249-3379 How to cite this article: Triyono T, Amijaya KAT. Regular donor characteristics, inter-donation interval and the presence of subclinical anemia – A 3-year observational single-center study. J Adv Pharm Educ Res. 2023;13(2):118-23. https://doi.org/10.51847/EQsMch0OCd