Workshops 36 W8 Visceral fat, adipocytolcines and cardiovascular risk W08-P-025 I INTERLEUKIN-6 AND TNF-ALPHA IN HEMODIALYSIS PATIENTS: ASSOCIATION WITH OBESITY AND METABOLIC SYNDROME Z. Rasic-Milutinovic 1, G. Perunicic 2, S. Pljesa 2, M. Ific 3, S. Stankovic 3, E. Stokic 4. ]Department of Endocrinology, University Hospital, Zemun, Serbia; 2 Departmant of Nephrology and Dialysis, University Hospital, Zemun, Serbia; ~Department of Medical Biochemistry UCC of Serbia, Beograd, Serbia; 4Clinic of Endocrinology, Diabetes and Metabolism, UCC Novi Sa~ Serbia Objective: We assessed the potential relationships between two inflamma- tory cytokines levels and insulin sensitivity of periferal tissue, in overweight hemodialysis patients (HD pts). Methods: Circulating concentrations of IL-6 and TNF-alpha (enzyme- linked immunoserbent assay), body composition (bioimpedance measure- ments), insulin resistance index (IR-HOMA) were measured in 42 HD pts without diabetes (23 men, 19 women). Results: Fasting plasma IL-6 and TNF-alpha were significantly higher in HD pts of I textile of body fat mass (FAT~o), or malnurished subjects, when compared with HD pts of III teltile, or overweight (IL-6 468.77 ± 414.99pg/ml vs 398.65 ± 335.15pg/ml, p=0.05; "IN'F-alpha 268.18 ± 198.86pg/m1). Plasma TNF-alpha was related to body fat mass (FAT~,o r=0.592, p=0.02; FAT ~ r=-0.593, p=0.02) only in overweight pts.Plasma IL-6 did not correlate with adipose tissue content in HD pts. The patients of III textile were with significantly higher IR-HOMA, plasma triglycerides, and lower HDL-cholesterol, but that metabolic parameters and IR-HOMA were not related to inflammatory cytokines. Conclusion: Our results showed that circulating levels of IL-6 and TNF-alpha were higher in malnurished HD pts, but adipose tissue in overweight subjects strongly correlated with plasma level of TNF-alpha and metabolyc sindrome. It seems that TNF-alpha concentration was not associated with insulin resistance in HD pts. I W08-P-026 I HEPATOCYTE GROWTH FACTOR MEDIATES INDUCTION OF PAI-1, IL-6 AND IL-8 IN HUMAN ADIPOSE TISSUE G. Rega z, C. Kaun 1, T.W. Weiss 1, S. Demyanets 1, M. Frey 2, G. Maurer 1, 3 1 1 K. Huber , J. Wojta . Department of Internal Medicine li, Medical University Vienna, Austria; 2Department of Surgery, Medical University Vienna, Austria; 5 3rd Department of Medicine, Wilhelminenspita~ Vienna, Austria Objective: Obesity is accociated with an increased risk of developing car- diovascular disease. The pro-atherogenic adipokines plasminogen ~tivator inhibitor- 1 (PAl-I), interleukin (I1)-8 and I1-6 are elevated in patients with obesity, insulin resistance and type 2 diabetes. On the other side serum hepateeyte growth factor (HGF) levels are also elevated in patients with atherosclerosis and obesity. In this study we investigate whether HGF regulates the expression of PAI-1, I1-6 and 11-8 in human adipose tissue. Methods: Primary human preadipocytes were prepared from adi- pose tissue. Differentiation to adipocytes was induced by hormone- supplementation. Preadipocytes and adipocytes were treated with HGF for 48h. PAI-1, I1-6 and I1-8 antigen were quantified by ELISA, mRNA levels were determined by RealTimePCR. Results: HGF significantly up-regulates PAI-1 production in both preadipocytes and adipocytes up to 9-fold and 6-fold, I1-6 production up to 18-fold and 13-fold and I1-8 production up to 6-fold. These results were confirmed on the level of mRNA expression. Conclusion: Our results demonstrate a significant upregulation of PAL 1, I1-6 and I1-8 expression in human preadiopcytes and adipocytes. If these effects are also operative in vivo one could hypothesize that elevated levels of HGF seen in obese subjects might contribute to the progression of atherosclerosis through upregulation of the adipokines PAI- 1, I1-6 and n-8. I wog-P-027 I APPLICATION OF A SIMPLE DEVICE TO ASSESS I i VISCERAL FAT AND PLASMA ADIPONECTIN MEASUREMENT FOR MANAGEMENT OF THE METABOLIC SYNDROME M. Ryo 1 , K. Maeda 2, M. Katashima3, T. Onda 3, M. Noguchi 4, K. Kishida 1, M. Nishida 1, T. Funahashi 1, T. Nakamura 1, Y. Matsuzawa 5, I. Shimomura 1. ~ Dept of lnternal Medicine and Molecular Science, Graduate School of Medicine, Osaka Univ, Suita, Japan; 2Medical Center for Translational Research, Osaka University Hospital, Suita, Japan; ~ Kao Corporation, Tokyo, Japan; 4Amagasaki Municipal OJ~ce, Amagasaki, Hyogo, Japan; 5 Sumitomo Hospital, Osaka, Japan Objective: Visceral fat accumulation is closely associated with coronary artery disease, and one of the major risk factors for the metabolic syndrome. Adiponectin is an adipose-derived plasma protein with anti-atherogenic and insulin-sensitizing activities. For management of the metabolic syndrome we measured plasma adiponectin concentrations and assessed visceral fat area (VFA) by bioelectrical impedance analysis (BIA). Methods: A current was applied between the umbilicus and the b~k and voltage was measured at the flank (abdominal BIA). The correlation between VFA presumed by abdominal BIA and VFA determined by CTwas investigated. The effects of physiological status on abdominal BIA were examined. Abdominal BIA and adiponectin measurement were applied in 3471 middle-aged adults who underwent health examination for assessment of the metabolic syndrome. The effect of short-term body weight reduction (BWR) therapy on fat accumulation and various metabolic parameters were investigated in 25 patients with diabetes meUitus, dyslipidemia and obesity. Results: The VFA presumed by BIA correlated significantly with VFA determined by CT. The correlation was significantly stronger than those between VFA determined by CT v.s. body mass index (BMI), waist circumference (WC), and relative body fat. The coefficient of variation of presumed VFA was within 7.1% in physiological status. The inci- dence of metabolic syndrome was higher in the group with high VFA or hypoadiponectinemia than in the group with < 100 cm 2 VFA or > 4.01xg/ml adiponectin. The BWR therapy improved metabolic parameters, and reduced VFA and presumed VFA by 20% and reduced weight, BMI, WC, and subcutaneous fat area by 5%. Conclusions: Application of abdominal BIA and measurement of plasma adiponectin may be useful for the metabolic syndrome management. I wog-P-028 I B-TYPE NATRIURETIC PEPTIDE AND ENDOTHELIN IN HIGH CARDIOVASCULAR RISK PATIENTS WITHOUT TARGET-ORGAN DAMAGE J. Saban-Ruiz, E. Bemal, O. Sanchez, J. Ma_rquez~ B. Monge, E. Fernandez, D. Coca, E. Sanchez-Largo, A. Trueba, R. Fabregate. Endothelial Pathology Uni~ Ramon y Cajal Hospital, Madrid, Spain Introduction: The main function of natriuretic peptides A, B and C, is that of antagonists of angiotensin II (AT II), but their relationship with other hormones or peptides has not been clarified. Among the natriuretic peptides, the Nt-pro-B-Type NP has been the most studied. On the other hand, ET-1 modulates the vasomotor tone, acting on two receptors: ETA (vasoconstrictor) and ETB (vasodilator). When a paradoxical vasodilating response appears in patients with high levels of ET-1, it has been attributed to the predominance of ETB receptors (Newby et al. J Am Coil Cardiol 1998), although this has not been confirmed. Objectives: 1 - To study the plasma levels of Nt-pro-BNP in a population of high cardiovascular risk, defined by the presence of at least two risk factors, without target-organ damage (TOD). 2 - To correlate them with the plasma levels of endothelin and the blood pressure response measured by 24-hour ambulatory blood pressure monitoring (ABPM). Methods: n=75, aged 35-85, 35 m, 50 hypertensive, 27 type 2 DM and 20 dyslipemic. Those with target-organ damage or severe hypertension measured by ABPM (>140/90 average) were excluded. TOD: Microal- buminuria, funduscopic examination, carotid Doppler, ECG (Minnesota code).ABPM: Spacelabs 90207. Blood pressure measures were taken at 20-minute intervals during both activity (9h at 22h) and rest (22h at 9h) pe- riods. Hemodynamic Load (HL) = Heart Rate x BPM/100. ENDO'IHELIN (fmol/ml): Bomedica ELISA (1-21) HRPO/TMB 96. Nt-proBNP (fmol/ml): Biomedica ELISA. Statistical analysis: t-student, chi-square. RESULTS: 1- The levels of Nt-proBNP were elevated (>230 fmol/ml) in 55.5% of the population. 2- The patients with high ET1 levels (>1.12) had a higher level of Nt-proBNP (p <0.05) and a lower HL (p <0.05). 75th EAS Congress, 23-26 April 2005, Prague, Czech Republic