_____________________________________________________________________________________________________ *Corresponding author: E-mail: chizaramwinnersndubueze@gmail.com; Journal of Advances in Medical and Pharmaceutical Sciences 23(11): 17-28, 2021; Article no.JAMPS.82603 ISSN: 2394-1111 Determination of Plasmid Profile Multiple Antibiotics Resistant Pseudomonas aeruginosa Isolated from Clinical Specimens in Healthcare Facilities across Anambra and Imo States Nigeria Seraphim Chinyere Ifegbuike a , Godwin Chukwuemeka Ojiegbe a , Henry Chidozie Amah a , Chizaram Winners Ndubueze a* , Joy Nkeiruka Dike-Ndudim a and Emeka Simon Anikwo b a Department of Medical Laboratory Science, Faculty of Health Sciences, Imo State University, Owerri, Nigeria. b Department of Human Physiology, Faculty of Basic Medical Sciences, Imo State University, Owerri, Nigeria. Authors’ contributions This work was carried out in collaboration among all authors. All authors read and approved the final manuscript. Article Information DOI: 10.9734/JAMPS/2021/v23i1130268 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/82603 Received 21 October 2021 Accepted 24 December 2021 Published 25 December 2021 ABSTRACT The emergence and dissemination of antibiotic resistant plasmids of P. aeruginosa is posing a major public threat and huge concern in hospital facilities. This study was done across Anambra and Imo States with a total number of 100 P. aeruginosa isolates, 50 from each State to determine the plasmid profile of multidrug resistant isolates. Methods of re-identifying P. aeruginosa were based upon cultural methods coupled with biochemical tests. To study the susceptibility of these isolates using disk diffusion method, seven (7) antibiotics were used namely: Piperacillin–tazobactam (100/10 μg), Ceftazidime (30 μg), Amikacin (30 μg), Ciprofloxacin (5 μg), Cefuroxime (30 μg), Ceftriazone (30 μg) and Gentamicin (10 μg). Plasmid extraction was done using alkaline lysis method after growing isolates resistant to more than four antibiotics on a nutrient broth. Agarose gel electrophoresis of plasmid DNA was carried out on 2% agarose gel slab in 1X TAE buffer. The results show that, the nutrient agar at 37˚C aerobically, P. aeruginosa isolates were recovered, Original Research Article