JOURNAL OF TROPICAL LIFE SCIENCE 2021, Vol. 11, No. 3, 375 – 382 http://dx.doi.org/10.11594/jtls.11.03.14 How to cite: Rafi M, Wulansari L, Septaningsih DA et al. (2021) Antioxidant Capacity and Phytochemical Profile of Punica granatum L. Peel Extracts Using Different Solvent Extraction. Journal of Tropical Life Science 11 (3): 375 – 382. doi: 10.11594/jtls.11.03.13. Research Article Antioxidant Capacity and Phytochemical Profile of Punica granatum L. Peel Ex- tracts Using Different Solvent Extraction Mohamad Rafi 1, 2* , Laela Wulansari 1 , Dewi A Septaningsih 1,3 , Tsania F Purnomo 4 , Reza Auliatifani 4 , Khaydanur 4 , Auliya Ilmiawati 2 , Wina Yulianti 4 , Nunuk K Nengsih 1 , Irma H Suparto 2,5 , Wisnu A Kusuma 1,6 1 Tropical Biopharmaca Research Center, Institute of Research and Community Services, IPB University, Jl Taman Kencana No. 3, Bogor 16128, Indonesia 2 Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jl Tanjung Kampus IPB Dramaga, Bogor 16680, Indonesia 3 Advance Research Laboratory, Institute of Research and Community Services, IPB University, Jl Palem Raya Kampus IPB Dramaga, Bogor 16880, Indonesia 4 Chemical Analyst Program, Vocational School, IPB University, Jl Kumbang No. 14 Kampus IPB Lodaya, Bogor 16128, Indonesia 5 Primate Research Center, Institute of Research and Community Services, IPB University, Jl Lodaya II No. 5 Kampus IPB Lodaya, Bogor 16128, Indonesia 6 Department of Computer Science, Faculty of Mathematics and Natural Sciences, IPB University, Jl Meranti Kampus IPB Dramaga, Bogor 16680, Indonesia Article history: Submission January 2020 Revised January 2021 Accepted April 2021 ABSTRACT Pomegranate is known to have valuable nutrients and contains various bioactive compounds found in the fruit's peel. The utilization of these bioactive compounds could be used as herbal medicines and supplements, such as antioxidants. This study aimed to determine the antioxidant capacity, phytochemical profile, and pomegranate peel extract grouping using different extracting solvents. The extraction solvents used were water, 70% ethanol, and ethanol pro analysis (p.a) grade. The antioxidant capacity of the three extracts was measured using DPPH and CUPRAC methods. We also determined the total phenolic and flavonoid levels and the TLC fingerprint analysis and FTIR spectrum of the pomegranate peel extracts. The 70% ethanol extract owned the largest antioxidant capacity. It was significantly different from the other two extracts with a value of 358.67 and 2981.59 µmol trolox/g dried sample using the DPPH and CUPRAC methods, respectively. The three pomegranate peel extracts' total phenolic and flavonoid levels ranged from 287.26–1068.81 mg GAE/g dried sample and 0.24-0.75 mg QE/g dried sample. TLC fingerprint analysis of pomegranate peel extract yielded 2, 6, and 6 bands for water extract, 70% ethanol, and p.a ethanol, respectively. The three extracts can be grouped based on FTIR spectrum data using principal component analysis using three principal components with a total variance of 93%. The results obtained showed that using different extracting solvents provides different antioxidant capacities and phytochemical profiles. Keywords: Antioxidant, FTIR spectra, Punica granatum, TLC fingerprint analy- sis, Principal component analysis *Corresponding author: E-mail: mra@apps.ipb.ac.id Introduction Pomegranate (Punica granatum L.) is a plant that belongs to the Lythraceae family, easy to grow in almost all climates spread in subtropical to tropical areas, from lowlands to highlands 1000 m above sea level. Several studies have reported that pomegranate has several biological activities,