Research Article The Effect of Pomegranate-Black Carrot Juice on Serum and ErythrocytesofSedentarySubjectsExposedtoExhaustingExercise Kursat Yusuf Aytac , 1 Ahmet Ozkaya , 2 Aykut Oztekin , 3 Vedat Cinar , 4 Taner Akbulut , 4 Ramazan Demirdag , 5 Veysel Comaklı , 5 and Kenan Bozbay 4 1 School of Physical Education and Sport, Adiyaman University, Adiyaman, Turkey 2 Department of Chemistry and Chemical Processing Techniques, Vocational School of Technical Sciences, Adıyaman University, Adıyaman, Turkey 3 Department of Medical Services and Techniques, Agri Ibrahim Cecen University Vocational School of ealth Services, Agri, Turkey 4 Faculty of Sports Sciences, Firat University, Elazig, Turkey 5 School of ealth, A˘ grı ˙ Ibrahim Çeçen University Department of Nutrition and Dietetics, A˘ grı, Turkey Correspondence should be addressed to Kursat Yusuf Aytac; kyaytac@gmail.com Received 10 April 2023; Revised 4 September 2023; Accepted 5 September 2023; Published 13 September 2023 Academic Editor: Juan D. Alch´ e Copyright © 2023 Kursat Yusuf Aytac et al. Tis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Background. In this study, the efects of pomegranate•black carrot juice mixture on serum and erythrocytes of sedentary in• dividuals who had exhaustion test were investigated. Methods. A total of 20 men voluntarily participated in the study. Blood samples were obtained from participants on three conditions. First, before the study, blood samples of participants were collected (baseline). Second, the same participants performed in the 20•meter shuttle run test for 1 week each day and were subjected to oxidative stress. Lastly, the same participants were given a mixture of pomegranate•black carrot juices (100 ml/100 ml) for a week, 45 minutes prior to the 20•meter shuttle run test, and the stress + supplement was performed. Blood samples were taken at the end of each process. Results. In the erythrocytes, while the oxidative stress condition malondialdehyde (MDA) level and carbonic anhydrase (CA) enzyme activity levels increased compared to the baseline, reduced glutathione (GSH) level, glutathione reductase (GR), and glutathione S•transferase (GST) enzyme activity levels decreased. In stress + supplement conditions, while GSH and GR levels increased according to oxidative stress conditions, CA and MDA levels decreased. While the lactate dehydrogenase (LDH) level of the oxidative stress condition increased compared to the baseline, the LDH level of the stress + supplement decreased compared to the oxidative stress condition. Conclusions. Our results showed that the level of oxidative stress in subjects exposed to the exhaustion test decreased with the mixture of pomegranate•black carrot juices. 1.Introduction Regular physical exercise plays an important role in the prevention of diseases such as cancer, obesity, depression, hypertension, and diabetes. Heavy physical exercises in• crease oxygen consumption through metabolism and ac• celerate the formation of reactive oxygen species (ROS) 1]. With the increase in oxygen uptake during exercise, free radical products are formed in the cell 2]. With the increase in exercise intensity, it causes more production of superoxide radicals 3], which are converted to hydrogen peroxide (H 2 O 2 ) by enzyme superoxide dismutase (SOD). H 2 O 2 can be detoxifed into water and oxygen by catalase (CAT) and glutathione peroxidase (GPx). H 2 O 2 is converted to hydroxyl radical via the Fenton reaction 4, 5]. After an acute exercise, the concentration of oxidative stress markers immediately increases in plasma 6, 7]. Severe physical exercise increases the production of ROS, negatively af• fecting the antioxidant system, and causes severe muscle pain. Furthermore, it causes an increase in the activity of Hindawi Journal of Chemistry Volume 2023, Article ID 9489018, 7 pages https://doi.org/10.1155/2023/9489018