Epidemiology and Society Health Review| ESHR Vol. 3, No. 2, 2021, pp. 1-9 ISSN 2656-6052 (online) | 2656-1107 (print) 10.26555/eshr.v3i2.3719 1 Research Article Entomological Index and Insecticide Susceptibility Status In Dengue-Endemic Areas Yogyakarta Fardhiasih Dwi Astuti 1 * and Arlina Azka 1 1 Faculty of Public Health, Ahmad Dahlan University, Yogyakarta, Indonesia * Correspondence: fardhiasih.dwiastuti@ikm.uad.ac.id. Phone: +6281328299534 Received 21 February 2021; Accepted 30 July 2021; Published 31 July 2021 ABSTRACT Backgrounds: Dengue Hemorrhagic Fever (DHF) is a disease that receives special attention because of causing outbreaks in some countries, including Indonesia. The problems that often emerge on the DHF Control Program are the high vector density (Aedes aegypti) and the vector resistance resulting from continuous insecticide use. This study describes the entomological index and susceptibility status of Aedes aegypti against malathion and permethrin insecticides in Sorosutan, Umbulharjo, Yogyakarta. Methods: This study used a descriptive observational method with a cross-sectional design. An entomological survey was done at 354 houses in Sorosutan, which was randomly selected. The sample of Aedes aegypti was obtained through the larval collection and ovitrap installation in 100 places. Susceptibility tests performed using the bioassay method with impregnated paper were based on the WHO standard. This study was conducted from December 2018 – February 2019. The data were analyzed using univariate analysis Results: The entomological survey in Sorosutan showed the House Index value was 35.03%; Container Index was 16.68%; Breteau Index was 46.33; Density Figure was 5.00, and Larva Free Rate was 64.97%; meaning that Sorosutan had a high level of vector density. So, the risk of DHF transmission was high. The population of Aedes aegypti in Sorosutan has been tolerant against Malathion, with 87.83% mosquito mortality. Conclusion: Sorosutan was an area with high DHF transmission risk. Aedes aegypti population in Sorosutan has been tolerant against Malathion. Keywords: Entomological index; Susceptibility status; Aedes aegypti; Malathion INTRODUCTION Dengue Hemorrhagic Fever (DHF) is a disease that receives concern globally because it continues to cause an outbreak in several countries. The incidence of DHF has increased rapidly worldwide in recent decades. It was estimated that as many as 390 million people per year were infected with DHF, of which 96 million people have clinical manifestations with a relatively high level of disease severity. In addition, as many as 3.9 billion people in 128 countries are at risk of being infected with the dengue virus (1).