242 SABRAO Journal of Breeding and Genetics 50 (3) 242-253, 2018 AGRONOMIC PERFORMANCE AND YIELD OF DOUBLED HAPLOID RICE LINES IN ADVANCED YIELD TRIAL A. HIDAYATULLAH 1 , B.S. PURWOKO 1* , I.S. DEWI 2 and W.B. SUWARNO 1 1 Department of Agronomy and Horticulture, Bogor Agricultural University, Jalan Meranti, Darmaga, Bogor, Indonesia 2 Indonesian Center for Agric. Biotech. & Genetic Resources Research and Development, Jalan Tentara Pelajar, Bogor, Indonesia *Corresponding author’s email: bspurwoko@apps.ipb.ac.id Email addresses of coauthors: akhmad_hidayatullah89@yahoo.com, iswari.dewi01@gmail.com, iswarisaraswati@pertanian.go.id, willy@ipb.ac.id SUMMARY Anther culture can be used to accelerate plant breeding program in producing superior variety of rice. The aims of this research were to determine agronomic performance and yield of doubled haploid (DH) lines of lowland rice and select the best genotypes for multilocation yield trials. The research was conducted in Indonesia at three locations, i.e. Subang (West Java), Cianjur (West Java) and Malang (East Java) using a randomized complete block design. It consisted of 35 genotypes and three check varieties (Ciherang, Inpari 13 and Inpara 5) as treatments. The treatments were repeated 3 times. The results showed that interaction of genotype and environment was significant on all traits. Genotypic variance and heritability estimates of all traits were in the high category and ranged between 1.57 – 458.51 and 0.58 - 0.96, except for tiller number and the productivity which were moderate. Fourteen DH lines were selected based on yield and selection index, which takes, into account a combination of traits, i.e. tiller number, filled grain per panicle, percentage of filled grain per panicle, 1000-grain weight, days to harvest and productivity, according to their relative weightage. The yield of the lines ranged between 4.82 - 6.60 ton/ha. Yield of Ciherang, Inpari 13 and Inpara 5 were 5.76, 4.85, and 4.82 ton/ha respectively. The selected DH lines will be used further in multilocation yield trial. Key words: Doubled haploid lines, genetic variance, heritability, selection index, yield trial Key findings: Fourteen doubled haploid rice lines with good agronomic performance and high yield were selected from advanced yield trials for further multilocation yield trials. They showed similar or higher productivity and weighted selection index value than three check varieties including Ciherang, a mega variety, which has the largest area of harvest in Indonesia. RESEARCH ARTICLE