Morphological and phylogenetic characterisation of novel Cytospora species... 93 Morphological and phylogenetic characterisation of novel Cytospora species associated with mangroves Chada Norphanphoun 1,2 , Olivier Raspé 3,4 , Rajesh Jeewon 5 , Ting-Chi Wen 1 , Kevin D. Hyde 2 1 Te Engineering Research Center of Southwest Bio-Pharmaceutical Resources, Ministry of Education, Guizhou University, Guiyang, 550025, China 2 Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100 Tailand 3 Botanic Garden Meise Nieuwelaan, 38, 1860, Meise, Belgium 4 Fédération Wallonie-Bruxelles, Service général de l’Enseignement supérieur et de la Recherche scientifque, rue A. Lavallée 1, 1080 Brussels, Belgium 5 Department of Health Sciences, Faculty of Science, University of Mauritius, Reduit, 80837, Mauritius Corresponding author: Ting-Chi Wen (tingchiwen@yahoo.com) Academic editor: A. Vizzini  |  Received 27 June 2018  |  Accepted 3 August 2018  |  Published 28 August 2018 Citation: Norphanphoun C, Raspé O, Jeewon R, Wen T-C, Hyde KD (2018) Morphological and phylogenetic characterisation of novel Cytospora species associated with mangroves. MycoKeys 38: 93–120. https://doi.org/10.3897/ mycokeys.38.28011 Abstract Mangroves are relatively unexplored habitats and have been shown to harbour a number of novel spe- cies of fungi. In this study, samples of microfungi were collected from symptomatic branches, stem and leaves of the mangrove species Xylocarpus granatum, X. moluccensis and Lumnitzera racemosa and examined morphologically. Te phylogeny recovered supports our morphological data to introduce three new spe- cies, Cytospora lumnitzericola, C. thailandica and C. xylocarpi. In addition, a combined multi-gene DNA sequence dataset (ITS, LSU, ACT and RPB2) was analysed to investigate phylogenetic relationships of isolates and help in a more reliable species identifcation. Keywords 3 new species, Cytosporaceae, Lumnitzera racemosa, Mangroves, Phylogeny, Taxonomy, Xylocarpus granatum, Xylocarpus moluccensis Copyright Chada Norphanphoun et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. MycoKeys 38: 93–120 (2018) doi: 10.3897/mycokeys.38.28011 http://mycokeys.pensoft.net A peer-reviewed open-access journal MycoKeys Launched to accelerate biodiversity research RESEARCH ARTICLE