How to Cite: Shebeeb, D. T., Shartooh, S. M., & Abed, S. M. (2022). Genetic relationship of clinical bacterial isolates with their environmental spreading using ERIC-PCR. International Journal of Health Sciences, 6(S4), 9625–9632. https://doi.org/10.53730/ijhs.v6nS4.12029 International Journal of Health Sciences ISSN 2550-6978 E-ISSN 2550-696X © 2022. Manuscript submitted: 9 April 2022, Manuscript revised: 18 June 2022, Accepted for publication: 27 July 2022 9625 Genetic relationship of clinical bacterial isolates with their environmental spreading using ERIC-PCR Duaa Tariq Shebeeb Department of Biology, College of Science, University of Anbar, Al-Anbar, Iraq Sufyan M. Shartooh Department of Biology, College of Science, University of Anbar, Al-Anbar, Iraq Suha M. Abed Department of Biology, College of Science, Tikrit University, Salaheldin, Iraq Abstract---The study examined the relationship between environmental bacterial and the pathological isolates of inward patients. It identified the biological safety level procedures followed. This work was conducted during the period from December2021 till May 2022 in Ramadi city (Anbar Governorate). Samples were divided into 30 environmental samples collected from the hospital environment, sewage and water, and 100 clinical samples collected from inpatients. These patients were in The Ramadi Teaching Hospital and in The Women’s and Children Hospital. Bacterial isolates were diagnosed using biochemical tests and confirming using VITEK2 technique. Some chemical and physical variables were detected. This work studied the Genetic fingerprinting of selected bacterial species by the Enterobacterial repetitive intergenic consensus of Polymerase Chain Reaction ERIC-PCR and conducted the dendrogram program was conducted for showing the relationships between environmental and pathological isolates of E. coli. As for the genetic fingerprint examinations environmental E. coli, the statistical analysis of the dendrogram program using ERIC-PCR technique had indicated that there was a similarity ratio 74.58% with the clinical E.coli. The study found that the hospital environment and the sewage water flowing from it are polluted to a large extent by pathogenic bacteria referring the weakness of biological safety procedures followed in the studied hospitals, which eventually might affect the quality of the environment. Keywords---E. coli, ERIC-PCR, biosafety.