*Correspondence: udayalaxmi68@gmail.com; +91 9342598080 (Received: June 22, 2020; accepted: September 22, 2020) Citaton: Varghese A, Udayalaxmi J, Rao P, Suman E. Blood Stream Infectons caused by Non-Fermentng Gram Negatve Bacilli, Clinical Correlaton, Mic for Colistn, Gene Detecton. J Pure Appl Microbiol. 2020;14(3):2017-2025. doi: 10.22207/JPAM.14.3.41 © The Author(s) 2020. Open Access. This artcle is distributed under the terms of the Creatve Commons Atributon 4.0 Internatonal License which permits unrestricted use, sharing, distributon, and reproducton in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creatve Commons license, and indicate if changes were made. Varghese et al. | J Pure Appl Microbiol | 14(3):2017-2025 | September 2020 Artcle 6436| htps://doi.org/10.22207/JPAM.14.3.41 Print ISSN: 0973-7510; E-ISSN: 2581-690X ReseARCh ARtiCle OPeN ACCess www.microbiologyjournal.org 2017 Journal of Pure and Applied Microbiology Blood Stream Infectons caused by Non-Fermentng Gram Negatve Bacilli, Clinical Correlaton, MIC for Colistn, Gene Detecton Angel Varghese, Jeppu Udayalaxmi*, Pooja Rao and Ethel Suman Department of Microbiology, Kasturba Medical College, Mangalore, Manipal Academy of Higher Educaton, Manipal - 576 104, Karnataka, India. Abstract To study the risk factors and outcome of blood stream infecton caused by non-fermentng gram negatve bacilli (NFGNB) and their patern of antbiotc susceptbility and genes. We included sepsis cases with blood culture positve for NFGNB. MIC for colistn was determined by broth microdiluton method. Multplex PCR was used to detect Bla IMP , Bla VIM , Bla KPC , Bla NDM-1 genes in cephalosporin and carbapenems resistant Acinetobacter spp. isolates. Out of 4,664 cases of sepsis, 50 (1.07%) were positve for NFGNB. Acinetobacter spp. 29 (58%) was the predominant isolate, of which 16 (55.17%) isolates were resistant to cephalosporins and carbapenems. We detected Bla KPC and Bla NDM-1 genes in two of these isolates. We did not detect Bla IMP , Bla VIM , Bla KPC and Bla NDM-1 genes in any other NFGNB isolates. Majority of the strains of Pseudomonas spp. showed sensitvity to all the antbiotcs tested. NFGNB sepsis patents with respiratory illness correlated well with fatal outcome (p <0.05; OR 21). More numbers of Acinetobacter spp. sepsis cases had fatal outcome (p <0.05; OR 12.83). NFGNB sepsis patents with respiratory illness and those which yielded Acinetobacter spp. correlated positvely with fatal outcome. We detected Bla KPC and Bla NDM-1 genes in two strains of drug resistant Acinetobacter spp. Keywords: Bacteremia, colistn, Drug resistance, Gram negatve bacilli, Risk factors